column chromatography


Also found in: Dictionary, Thesaurus, Medical, Legal, Wikipedia.
Related to column chromatography: Thin layer chromatography

column chromatography

[′käl·əm ‚krō·mə′täg·rə·fē]
(analytical chemistry)
Chromatographic technique of two general types: packed columns usually contain either a granular adsorbent or a granular support material coated with a thin layer of high-boiling solvent (partitioning liquid); open-tubular columns contain a thin film of partitioning liquid on the column walls and have an opening so that gas can pass through the center of the column.
References in periodicals archive ?
The remainder was purified by silica gel column chromatography with ethyl acetate and hexanes as a mobile phase.
By combining ethanolic extraction with precipitation of impurities by diethyl ether and column chromatography on polyamide carrier, resveratrol with a small content of viniferin can be separated from lignified vine stems.
1), three late-pregnancy and three follicular fluid samples were subjected to additional Sephadex LH-20 column chromatography in methanol-toluene (9:91 by volume) to separate free estradiol from estrone and estriol (23).
To obtain bio-active compounds representing the neuro-protective activity of the n-BuOH fraction, this was further subjected to repeated column chromatography.
Nevertheless, the fact that Hb E resolves completely from Hb A in both anion and cation exchangers renders column chromatography an invaluable tool for the screening of Hb E in both adults and newborns [13,141.
The global chromatography systems market is segmented into the following categories: Chromatography Systems Market, by Types Gas Chromatography Liquid Chromatography High Pressure Liquid Chromatography (HPLC) Ultra High Pressure Liquid Chromatography (UHPLC) Low Pressure Liquid Chromatography (LPLC) Others Ion Exchange Chromatography (IEC) Affinity Chromatography (AC) Supercritical Fluid Chromatography (SFC) Column Chromatography Thin Layer Chromatography (TLC)
Contract award: 4 automatic flash column chromatography system.
It also introduces the concept of chromatography and compares HPTLC with column chromatography.
The differences between their results and ours may be the higher recovery of testosterone and specificity of the extraction (ethyl acetate/hexane)-chromatography RIA assay vs an extraction (ethyl acetate) RIA without a column chromatography purification step, especially in serum samples containing a high concentration of testosterone.
Traditional column chromatography dominates current purification technology, and many of the productivity gains that have been achieved have relied on upscaling such devices.