- Advancements in Molecular Cloning Drives Competent Cell
The double-enzyme digestion vector and the annealed double-stranded DNA were transformed into competent cell
with T4 DNA ligase.
In addition, the Venn diagram revealed that the differences observed between CTC-MCC-41 and HT-29 do not just reflect of differences between 2 different cell lines, but were exclusive to the CTC-MCC-41 metastasis competent cells
. Additional studies of other yet-to-be-characterized metastasis competent cell
lines will be necessary to determine whether these differences are unique to metastasis competent cell
lines in general.
Intermediate cloning vector pEASY-T Simple, competent cell
TOP 10 and high fidelity Trans Taq-T DNA polymerase were purchased from Transgen Biotech Company.
After that continue the centrifugation step as above, the resulting cell pellet was resuspended in one-tenth volume (4 ml) of sterile cold TB (Ca[Cl.sub.2]) solution to yield the final competent cell
During the heat shock period the motion of tiny plasmid DNA molecules in the competent cell
mixture is likely to increase.
Almost any competent cell
biologist or geneticist can attempt to produce human iPS cells.
The United States District Court of the Western District of Texas (Austin Division) issued a summary judgment ruling on November 2, 2001 in favor of Stratagene (La Jolla, CA; 858-592-9900), holding that Stratagene's chemically competent cell
products do not infringe Invitrogen's U.S.
The yeast strain used in this study was the YRG-2 competent cell
line (MATa ura3-52 his3-200 ade2-101 lys2-801 trp1-901 leu2-3 112 gal4-542 gal80-538 lys2::[U A S.sub.GAL1] - [T A T A.sub.GAL1]-H I S 3 URA3::[UAS.sub.GAL4 17mers (x3)]-[TATA.sub.CYC1]-Lac2) provided by Stratagene (Basel, Switzerland).
The purified PCR product was ligated with the pDrive cloning vector and then transformed into the QIAGEN EZ competent cell
. Then, the competent cell
was transferred to the LB medium that contained kanamycin (antibiotic), X-gal and IPTG.
The purified c-FLIP(L) genes were ligated with pMD19-T vector (Takara, Dalian, China) and then were tranformed into the competent cell
Key words: competent cell
; IBDV-vp2; electroporation; lactobacillus; transformation; ameliorate maneuver