The complement fixation
method is more sensitive than the immunodiffusion test and presents variable test sensitivity, depending on the antigen phase, ranging from 72.
Because hyphal forms are rarely encountered in tissue, a correlation between complement fixation
titers and immunodiffusion result patterns has not been established in the literature.
Evidence supporting complement fixation
as the basis of ENL reactions is essentially indirect, derived from patients' sera and biopsied tissues.
Progressive loss of efficiency and fatal outcome resulting in massive economical losses forced veterinary authorities worldwide to start disease control including mass testing using complement fixation
test and/or malleinisation, and culling of positives.
for Coccidioides was attempted but was non-diagnostic due to anti-complement activity.
Serum samples were submitted to complement fixation
test (CFT) according to literature (RASO et al.
For the investigation of fever of infectious origin, brucellosis, leptospirosis, toxoplasmosis, HTLV1, HTLV2, and viral hepatitis types A, B, C were investigated, and ELISA method was used for all research; for mononucleosis, another two samples were collected, and the method used was Hoff and Bauer; for leishmaniasis and paracoccidioidomycosis the technique of complement fixation
reaction was used; for Chagas disease, immunofluorescence was used; for cytomegalovirus, chemiluminescence was used.
capsulatum, Blastomycosis dermatitidis, and Paracoccidioides brasiliensis by using complement fixation
It may be safe if patients continue fluconazole and as long as their complement fixation
titer remains negative.
EVALUATIONS/MEASUREMENTS: We defined a case by positive immunodiffusion serology, a [greater than or equal to] 4-fold titer rise in complement fixation
between acute and convalescent sera, or positive urinary Histoplasma capsulatum (HC) antigen.