Results of RT-PCR and complement fixation
This method is more specific than the complement fixation
(2,3) and presents the following strengths: (i) it is based on simple and reliable methodology; (ii) it has a low cost; (iii) specificity is close to 70-100%.
Antibodies against Varicella virus were detected using complement fixation
test (CFT) and Immunoglobulin M (IgM) by ELISA.
Serology for H capsulatum, obtained for the evaluation of fever of unknown origin, revealed mycelial and yeast complement fixation
titers of 1:512 with a positive M and H band on immunodiffusion.
leprae suspensions were incubated with normal serum and a hemolytic titer (CH50) was determined as a measure of complement fixation
. Crude M.
Rheumatoid factor, anti-nuclear antibody, anti-cardiolipin antibody, complement fixation
, and serum protein electrophoresis were all within normal limits.
Serologic testing using both a complement fixation
assay and immunodiffusion assay is generally the best way to diagnosis histoplasmosis.
A complement fixation
assay may not show positive results until 2-6 weeks after onset of acute illness, and an immunodiffusion assay often lags further behind.
They realized that the simplicity of EIA/ELISA compared very favorably with the cumbersome technology that was used in the field at that time (immunofluorescence, hemagglutination inhibition, and complement fixation
) and quickly developed a wide range of tests for various microorganisms.
Documented methods of VZV antibody detection include complement fixation
, IFA, fluorescent antibody to membrane antigen (FAMA), neutralization, indirect hemagglutination, immune adherence hemagglutination, radioimmunoassay, latex agglutination, and enzyme-linked immunosorbent assay (CDC 1996).