SMC1/3 form the core of the cohesin complex which mediates sister chromatid cohesion; SMC2/4 are present in the condensin complex that acts in chromosome assembly and segregation.
Furthermore, since the Nesprin-2 condensin interaction also takes place during other phases of the cell cycle and since condensins have additional functions in interphase, the Nesprin-2 condensin complex could also affect these processes.
Carroll et al., "Mutations in genes encoding condensin complex proteins cause microcephaly through decatenation failure at mitosis," Genes & Development, vol.
As the chromosomes condense, REC8 is cleaved on the chromosome arms and the cohesin complex is replaced by the condensin complex
. The crossover point remains to ensure proper alignment, tension, and segregation of the homologous chromosomes.
I plan to use a multidisciplinary approach, combining acute protein inactivation, 4D-live cell imaging and biophysical/mathematical approaches to evaluate role of condensin complexes
, one of the most abundant non-histone chromosomal proteins, in the process of chromosome assembly.