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Anaemia is ruled out by the absence of pallor of the conjunctivae, face, mucous membranes, palms and haemoglobin estimation is not carried out on a routine basis in our selected recruits.
Hematoxylin- and eosin-stained slides were prepared from formalin-fixed, paraffin-embedded samples of the central nervous system, conjunctivae, tongue, salivary glands, lungs, heart, liver, gastrointestinal tract, spleen, adrenal glands, kidneys, and lymph nodes.
All children were examined biannually, and the conjunctivae of a stratified random sample of children were swabbed and later tested for Chlamydia trachomatis DNA.