Recently it was confirmed that consensus sequences
forming stable G-quadruplex structures are responsible for RNA replication and inhibition of protein translation of hepatitis C virus .
Partial sequences from the NCBI DNA sequence database that match segments of the consensus sequence
shown in Fig.
Briefly, consensus sequences
from the WSB, PWK, and CAST strains were mapped to the alignment and gaps filled with Ns.
Two individuals were found to have an identical consensus sequence
for the genotyped C*14:02:01 allele, which harbors 2 distinct polymorphisms, resulting in the reporting of only a single novel allele sequence.
RT-PCR was performed to confirm our speculation about the FAMLF consensus sequence
. Two peripheral blood samples from AL patients were amplified by RT-PCR and a specific band of expected size (2214 bp) was obtained, as well as an unexpected 429-bp band (Figure 1).
According to the results of multiple alignments of all the obtained consensus sequences
, it was possible to detect differences between the species T.
The higher the depth of read coverage, the better statistical strength this consensus sequence
has, as correct reads at any individual position increasingly outnumber individual read errors.
In Escherichia coli the SOS gene network is under control of the RecA protein which, as consequence of DNA damage, induces the autoproteolysis of LexA repressor which binds the 16 bp palindromic consensus sequence
named LexA-binding box.
The alignment file created is then used to construct a consensus sequence
using the IUPAC degenerate nucleotide codes .
The major difference is that it begins with a consensus sequence
containing degenerate bases and selects primers with fewer than 3 or 4 degenerate bases, so that in the end a majority of strains are amplified, but it does not require primers to amplify all strains.
This T-cell epitope is observed in all the serological variants of DEN, with each serotype having its distinct consensus sequence
for the epitope.