(A) Through analysis of a bioinformatics website, target genes complementary to the miR-375 conserved sequence
Immediately following the second HKD motif and in the middle of the highly conserved sequence
IGSANINQR, there is an invariant serine residue.
Several conserved sequence
blocks (CSBs) were located at the central conserved region and at the right hypervariable domain (Figure 2).
The 29 amino acid residues long motif "1" "DDPAIWVHPHDPEKSRIIGTNKKSGLAVY" was observed in all 44 protein sequences, with a conserved sequence
"DDPAIW[VI][HN]PK[DN]P[ESA]KS." This sequence might be used for diversity and expression analysis of BPPhy enzymes.
The molecular organization showed to be coincident with the consensus molecular structure described for other rodent taxa, exhibiting the conserved domains ETAS (extended termination-associated sequences), CD (central domain) and CSB (conserved sequence
block) 1, 2 and 3.
Hammerhead ribozymes, as based on the Haseloff and Gerlach model (Nature, 1988), consist of a catalytic core made up of a highly conserved sequence
of 24 nucleotides that cleave substrate RNAs.
In addition to hits found for CueO matching multicopper oxidases/laccases, the hits revealed that the conserved sequence
is also prevalent in proteins as blood coagulation factors V and VIII, Yeast FETIII involved in iron uptake, and yeast fission homolog SpAC1f7.08.
The mannose binding sites are based on conserved sequence
The sequence of splice sites in human is heterogenous, and thereby switching the splice sites into the conserved sequence
promotes splicing reaction.
We mapped variability onto this sequence rather than the artificial conserved sequence
for two reasons: first, conserved sequences
derived upon a MSA tend to be too long and contain stretches of amino acids not present in any real sequence, and secondly, AAA45676.1 has long been used as a reference sequence to design overlapping peptides and check T-cell responses.
These reagents contain shorter highly conserved sequence
regions improving assay specificity, and the probes are non-cleavable which allows post-PCR melt curve analysis.
By comparing with the recognition sites among Coilia species, three conservative domains were detected: the extended termination associated sequence domain (TAS), the central conserved sequence
block domain and the conserved sequence
block domain (Fig.