In the present study, the transgene delivered was under the control of CaMV35S promoter since it is a strong and constitutive promoter
(Seternes et al., 2016).
Quantification of Constitutive Promoter Activity Using GFP and luxCDABE Reporters.
We analyzed four constitutive promoters available in the Registry of Standard Biological Parts and a wild-type Plac promoter as regulated system.
In addition, novel target cells have been developed that express a constant high level of the specific antigen recognized by a particular monoclonal antibody under the control of a strong constitutive promoter as well as the homologous control target cells in which the gene encoding the specific antigen has been inactivated by genome editing .
Jurkat cells were then transfected sequentially, using the Neon transfection system (ThermoFisher Scientific, France) according to the manufacturer's recommendations, with the FL reporter gene construct, an expression vector for Fc[gamma]RIIIA, V-variant (InvivoGen, San Diego, CA) or the F-variant synthetized using standard techniques and inserted into the pUNO1 plasmid (InvivoGen, San Diego, CA), and the Nano-Luc[R] luciferase (NL) reporter gene under the control of a constitutive promoter. Stable clones were isolated and characterized for ADCC activity in the presence of a given therapeutic antibody and the appropriate target cells giving rise to the clonal iLite[TM] effector cell lines.
Results for all the experiments confirmed strong constitutive promoter
activity of C1 rep in monocotyledons plants as well.
For Agrobacterium tumefaciens-mediated transformation, strain LBA4404 harboring a T-DNA containing the translational fusion gene cry1Ab-1B driven by the maize ubiquitin constitutive promoter
along with its first intron and untranslated exon (Christensen and Quail, 1996) and bar (coding for phosphinothricin acetyltransferase) as the selectable marker gene, under the control of the 70S promoter (double enhanced constitutive 35S promoter from Cauliflower mosaic virus), (Fig.
 quantified a set of constitutive promoters
and found that activity was affected up to 4-fold when a specific upstream (UP) sequence is placed before promoters, even though in some cases activity was not affected.
Compositions are novel nucleotide sequences for inducible and constitutive promoters
isolated from a family of maize genes encoding pathogenesis-related proteins.
Activity of constitutive promoters
in various species from the Liliaceae.
At present, constitutive promoters
, including those of the cauliflower mosaic virus 35S transcript (Ou-Lee et al., 1986; Battraw and Hall, 1990), the rice Actin-1 gene (Zhang et al., 1991), and the maize Polyubiquitin-1 gene (Christensen and Quail, 1996), are widely used for expression of candidate antifungal genes in wheat and barley.
Whereas the constitutive promoters
such the CaMV 35S and nos gene promoters have been shown to function in linseed , the seed specific napin promoter is not effective .