The model was tested in two different human cell lines: MCF-7, an estrogen responsive cell line derived from metastatic human breast adenocarcinoma, which is known to express PTGS2 (12), and HEK293FT, a highly transfectable clonal isolate derived from human embryonal kidney cells HEK293, which does not constitutively
express PTGS2 (13).
High expressions of mRNA as the genes were driven by a constitutively
expressing 35S promoter.
The results revealed that the enzyme can express constitutively
Whether suppression of constitutively
active STAT3 in FaDu cells by resveratrol leads to apoptosis was also investigated.
Antimicrobial compounds of low molecular mass are constitutively
present in insects: characterization of a-Alanyl-Tyrosine.
However, if the gene encoding my transcription factor, which should exist in the cDNA library, is fused to the gene encoding the GAL4 activation domain, which is made to be constitutively
active, my factor should become constitutively
active in a yeast strain regardless of the presence or absence of ER stress.
The most common K-ras codon 12 mutations result in the substitution of glycine for valine, thus changing the spatial conformation of RAS, leading to a constitutively
activated form where the RAS signalling cascade to other participants occurs regardless of EGFR status.
sakazakii appears to constitutively
express high levels of a-glucosidase.
The second group of structural biomarkers includes proteins/enzymes constitutively
expressed in the tubules and released from injured cells into the urine.
The company offers not just plasmids for CRISPR gene editing, but also custom-guide RNA design and validation (to provide customers with confidence that they are working with a suitably active guide); custom donor design and manufacturing; QuickStart cell lines that constitutively
produce Cas9 nickase; and more than 550 X-MAN cell lines ready for further modification.
But the Ellis Island we encounter in Keith's study is less a port of access to an exceptional state than a place of imprisonment and expulsion, a symbol for how 'the process of becoming American is inextricably linked to the condition of unbecoming American' given that 'the universalism of American citizenship is constitutively
linked to the production of subjects excluded from national membership' (p.
In insects, AMPs are constitutively
expressed or highly inducible in response to bacterial infection by various epithelia of midgut and salivary glands, fat bodies and hemocytes in which they are secreted into the hemolymph.