For the evaluation of antibacterial activity against planktonic bacteria, 100 [micro]L of each well from the direct contact inhibition
assay (n=3) were vortexed in 900 [micro]L of saline solution (0.9%), diluted until [10.sup.-6] dilution, and platted in brain-heart infusion agar Petri dishes as previously described.
Since ADT is capable of determining antibacterial activity of only soluble agents which diffuse into agar gel and inhibit the bacterial growth, but is not appropriate for non soluble antibacterial agents with surface contact inhibition
Hypersensitivity to contact inhibition
provides a clue to cancer resistance of naked mole-rat.
(4,5) The cytoplasmic protein [beta]-catenin (14/[beta]-catenin, BD Biosciences, Erembodegem, Belgium) was evaluated as a first potential candidate because it is a low-molecular weight (88 kDa versus 660 kDa of thyroglobulin) peptide that is involved in adherens junctions, epithelial contact inhibition
signaling, and gene transcription.
Finally, cell growth ceased as a result of contact inhibition
from the day 5.