In order to have a bona fide continuous cell line it usually takes long time (sometime months) and it needs lot of patience and practice because sometime a cell line is established but it does not meet the criteria of true continuous cell line.
The usual criteria of continuous cell line includes altered cytomorphology reduced serum dependency increased clonogenicity tendency towards anchorage-independent growth change in the ploidy of the genome tumourigenicity in nude mice increased growth and an infinite life span of cells (Freshney 1992).
Using immobilized cells in meat starter cultures might make possible the production of a continuous cell line
, which ensures retention of stable cell lines; the prevention of unpredictable genetic alterations associated with restarting culture broths; and the continuous release of microorganisms to a nonsterile meat product that cannot be pasteurized, like liquid food products.
HepaLife's PBS-1 cells are a continuous cell line
which has already successfully lived through hundreds of passages.
Among these features are the peculiar tropism of the virus for Vero cells (a continuous cell line
established from monkey kidney epithelial cells), its capacity for growth at 37[degrees]C (while other respiratory coronaviruses grow at lower temperatures), and its ability to infect lower respiratory tract tissues (13).
Solvay Pharmaceuticals was the first influenza vaccine provider in the world to receive approval from a regulatory authority for continuous cell line
technology used in the prevention of influenza.
Cytopathic effect and plaque formation by arboviruses in a continuous cell line
(XTC-2) from the toad Xenopus laevis.
According to Barath Palada, a lead analyst at Technavio, specializing in research on infectious and rare diseases, The technological advances in the last few years such as the growing usage of continuous cell lines
as substrate and the approval of fermenter technology for antigen productions by USDA are helping in further consolidation of the vaccine market in the region.
Using mosquito continuous cell lines
, we recovered 49 DENV-3 isolates from confirmed case-patients.
continuous cell lines
such as Chinese hamster ovary (CHO) and myeloma cells (NSO and SP2/0)