To examine whether the observed defects in midzone assembly impacted furrow formation or assembly of the actin-myosin contractile ring during cytokinesis.
1998, pavarotti encodes a kinesin-like protein required to organize the central spindle and contractile ring for cytokinesis.
Activated myosin II in the contractile ring serves as an indicator of active RhoA (Jordan and Canman, 2012), suggesting that the RhoA-based furrowing induction system was being stimulated in activated eggs.
MgcRacGAP controls the assembly of the contractile ring and the initiation of cytokinesis.
RhoGEF and Rho family GTPase-activating protein complex links the contractile ring
to cortical microtubules at the onset of cytokinesis.
Therefore the observation that actin filaments self-organized and moved in an anti-parallel fashion in these extracts fits current models of the contractile ring
A similar sliding motion produced by bipolar myosin-II filaments is generally accepted as the mechanism by which the contractile ring
constricts the cell during cytokinesis.