There were significantly more VEGF+ cells in the coronal pulp of immature teeth than those of mature teeth (P=0.04), but there was no significant difference in the expression in the middle regions (P=0.27).
The total VEGF expression in the apical papilla was not significantly different from the total amount of protein observed in the coronal pulp (P=0.17).
VEGFR2 was expressed in the apical CRZ and the apical papilla where the expression was approximately half of that of the coronal pulp in immature teeth (Table 2).
The increased vascularity and angiogenic activity observed in the coronal pulp compared with the radicular pulp for both tooth types may in part explain the favourable healing that frequently follows stepwise caries excavation and direct pulp capping (23,27).
The coronal pulp
cavity index: A forensic tool for age determination in human adults.
The pulp chamber was opened with a sterile high speed diamond bur and the coronal pulp tissue was removed by a sharp spoon excavator and the chamber was then irrigated with sterile saline solution.
Group 2--After coronal pulp removal, a cotton pellet was dipped in ABS solution, squeezed dry and placed on the pulp tissues for 10-15 seconds then observed for complete haemostasis during one minute.
In this study, 1:5 dilution of Buckley's FC was used and the coronal pulp chamber was filled with ZOE according to the clinical guidelines presented by AAPD .
were removed and processed for indirect immunofluorescence using antibodies raised against TRPV1 and either the general neuronal marker, protein gene-product 9.5 or alpha smooth muscle actin in conjunction with Ulex europaeus agglutinin 1 lectin to fully label the pulp vasculature.
The teeth of patients in Group F were treated by coronal pulp amputation followed by a five minute application of a 20% Buckley's formocresol solution while the teeth of patients in Group C were treated by coronal pulp amputation hemostasis followed by placement of calcium hydroxide powder containing 99% calcium hydroxide (Sigma-Aldrich Chemical Company Ltd Poole Dorset UK) to the radicular pulp stumps.
Coronal pulp amputation was achieved with small and medium slow-speed sterile round burs taking care to avoid cutting the pulp chamber floor.
Partial pulpotomies are preferable to cervical pulpotomies in molar teeth as they allow preservation of the cell rich coronal pulp
tissue, providing a better healing potential; physiologic apposition of dentine in the cervical area is maintained (which is lost and dentinal walls weakened in cervical pulpotomy), a subsequent root canal treatment is not usually necessary but if required access can be gained more readily to the canals.