The midpregnant group was formed with pregnant individuals whose ovaries exhibited, at the time of sacrifice, ovulatory stigmatas as evidence of a recent ovulatory event and later confirmed for the presence of secondary corpora lutea
in hematoxylineosin stained ovary sections.
% Total rats 6 100 12 100 Mortality rate 0 0 6 *(a) 50 Total number of 39 50 *(a) corpora lutea
(in metrial gland) Resorptions 1 2.6 12 *(a) 24 Total number of fetuses 38 97.4 38 76 Live fetuses 38 100 31 81.6 Dead fetuses 0 0 7*a 18.4 Group Parameter STZ-DM + Zn STZ-DM + In No.
Embryonic loss, measured by the difference in mean number of corpora lutea
and mean number of fetuses, was 32.14%.
Supplementary corpora lutea
(SCL) formation was also monitored from day 35 of pregnancy onwards to evaluate a potential effect on fetal growth in Altrenogest group.
We cut ovaries into 1 mm sections and examined them under a 25x dissection microscope for the presence of corpora lutea
. We distinguished corpora lutea
from corpora albicantia by size and color (Hamilton and Eadie, 1964).
No test item-related influence on prenatal foetal development including number of corpora lutea
, implantation sites, resorptions and live foetuses, were detected at any of the EGb761[R] doses.
Lishman et al., "Maintenance of pregnancy in postpartum beef cows that have short-lived corpora lutea
.," Journal of animal science, vol.
from cyclic mares were collected post-mortem from April until the end of July at a local abattoir from randomly selected cyclic Lusitano mares.
The fetuses were removed and the following examinations were performed: macroscopic inspection of the placentae, the number of fetuses (alive and dead), the number of placentae, sex and viability of fetuses, number and size of resorptions, number of corpora lutea
, number and location of implantations and fetuses in the uterus, gravide uterus weight, and weights of the placentae and fetuses.
Although ovarian follicular atresia and luteal regression are normal physiological processes for the adequate maintenance of ovarian functions, the aberrant increase in these processes can cause disturbances in follicle selection and in the life span of corpora lutea
. Several in vitro studies support our findings: BPA treatment resulted in decreased E2 production in FSH-stimulated porcine (Mlynarcikova et al.
Thus, it is also possible that at the time of blood collection matured Graafian follicles had ovulated, forming corpora lutea
on the ovary resulting in increased serum progesterone concentrations.
Measurements included age and field-dressed body weight of both sexes, number of corpora lutea
(CL) and ovulation rate of females, and antler beam diameter (ABD) and antler spread of males.