Under injection thiopentone sodium anesthesia, overnight starved rats were fixed on rat operating table and hair in the axillae were clipped enough to implant 2 sterile cotton pellets
, through the small incisions made subcutaneously on both the sides, aseptically.
Inter-appointment moist cotton pellet
is required till the final setting of MTA, difficulty in removal after curing due to the absence of a known solvent for set MTA, high cost.
In a previous study, although shikonin accelerated collagen fiber formation in the granulation tissue in the study by cotton pellet
method,[sup] it was considered to be due to increase of fibroblasts number.
penicillata (400 mg/kg) and indomethacin (10 mg/kg) on cotton pellet
induced granuloma as a model of chronic inflammation; significant difference results (P < 0.
The 3% potassium oxalate gel (Oxa Gel, Art Dent, Araraquara, SP, Brazil) was applied to the dentin surface with a cotton pellet
and left undisturbed for 2 minutes.
Table 1: Effect of Strychnos nux vomica on cotton pellet
The cotton pellet
method as described by Winter CA, Porter CC with slight modification has been used for granuloma formation.
The present results indicate a significant anti-inflammatory activity of PPG isolated from Wattakaka volubilis in cotton pellet
granuloma and found to be effective in chronic inflammatory conditions, which reflects its efficacy in inhibiting the increase in the number of fibroblast and synthesis of collagen and mucopolysaccharides during granuloma tissue formation as reported by Recio et al.
In sub-acute model of cotton pellet
test, test drugs were found to be efficacious.
Table-2: Effect of solvent fractions of ethanol extract of Crotalaria burhia root on cotton pellet
induced chronic inflammation in rats Treatment Wet weight Dry weight (mg/kg) DMSO + 950.
The carrageenan-induced paw edema, cotton pellet
granuloma and indomethacin-induced gastric damage models were used to determine these effects.
Key words: Inflammation, antiinflammatory, Hedera helix, ivy plant, carrageenan, cotton pellet