counterstain


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counterstain

[′kau̇nt·ər‚stān]
(biology)
A second stain applied to a biological specimen to color elements other than those demonstrated by the principal stain.
References in periodicals archive ?
4) the nuclear/ cytoplasmic differentiation was markedly reduced and the counterstain seemed to stain all the structures uniformly resulting in the so-called "pink disease".
The cells were then rinsed with dH20, and, if desired, immediately covered with 100 [micro]l methyl green counterstain solution and incubated for 20 minutes.
The present study was undertaken with the objective to stain sputum samples in their containers by 'phenol (10%) ammonium sulphate (4%) basic fuchsin (2%) solution' and to decolourize and counterstain their smears for detection of AFB- (henceforth called pot method) and to compare the smear results of pot method with the standard Ziehl-Neelsen (ZN) method.
These open pores allow the counterstain safranin to pass through the cell wall.
Purified methyl green served as a cytoplasmic and nuclear counterstain.
Propidium iodide (PI) was used as a counterstain and fluorescein isothiocyanate (FITC)-conjugated avidin DCS was used to detect the probe.
A drop of detection reagent was placed in each well, and a drop of counterstain was added to each well.
The solvent bleaches gram-negative bacteria, which microbiologists can then detect using a pink counterstain.
DAPI staining (Beyotime Institute of Biotechnology, China) was used as nuclear counterstain for the fluorescent quantification of DNA content.
The Leica Bond Polymer Refine Red Detection System (Leica Microsystems, Wetzlar, Germany) was used at manufacturer's specifications, including deparaffinization, enzyme retrieval, primary antibody incubation (DAKO CK AE1/3, 1:200 for 15 minutes), Polymer Red detection, and hematoxylin counterstain.
Sections of ovaries that were 5 [micro]m thick were mounted and stained with Harris's hematoxylin followed by eosin counterstain.