After 72 h of culture, (A) representative images are shown of cells fixed and subjected to fusion assay [VCT and ST nuclei were immunostained with anti-TWIST1 (marker of ST, yellow) and anti-DESMOPLAKIN (red) antibodies and counterstained
with DAPI (blue)].
The sections were counterstained
The slices counterstained
with DAPI were consistent with these results.
Frozen sections, immunohistochemistry counterstained
Positive control: CD31 (red) counterstained
with DAPI (blue) in the muscle of mouse; vimentin (green) counterstained
with DAPI (blue) in the testis of mouse.
The suppressor DNA control experiments showed complete absence of hybridization signals on the propidium iodide counterstained
metaphases (Supplementary Figure 2).
Caption: Figure 6: Confocal immunofluorescence microscopy of caspase-3 (red fluorescence) using an antibody detecting pro-caspase-3 recombinant protein (A-D) or cleaved caspase-3-recombinant protein (E-H) with DAPI- counterstained
nuclei (blue fluorescence) in the kidney sections of control or ACF adult rats.
For immunohistochemistry, paraffin-embedded sections were stained with rabbit anti-nytrotyrosine primary antibody (Upstate, number 06284, 1: 200), rabbit anti-CD3 antibody (DAKO, number A0452), and rat anti-F4/80 antibody (AbD Serotec, number MCA497R) then the staining was revealed with Histofine reagent (Nichirei Biosciences) and slides were counterstained
Finally, the sections were washed in PBS and counterstained
Nucleus was counterstained
with 4,6-diamidino-2phenylindole (DAPI, Beyotime Institute of Biotechnology, China) for 15 min.
The slides were rinsed as mentioned earlier and counterstained
with 0.1% methylene (MB) blue for 30 s.
(B) The cells were immunostained with anti-E- cadherin, anti-N-cadherin and anti-Fibronectin antibodies, and nuclei were counterstained