counterstain

(redirected from counterstaining)
Also found in: Dictionary, Thesaurus, Medical.

counterstain

[′kau̇nt·ər‚stān]
(biology)
A second stain applied to a biological specimen to color elements other than those demonstrated by the principal stain.
References in periodicals archive ?
Immunoreactions were detected by the labeled streptavidin-biotin method and visualized with 3, 3-diaminobenzidine, followed by counterstaining with hematoxylin.
On the second day, after several washes with phosphate buffered saline, the sections were incubated with biotinylated rabbit anti-rat secondary antibody and avidin-biotin horseradish peroxidase (Vector Elite Avidin Biotin Complex system, Vector Laboratories, Burlingame, CA) for 30 minutes, respectively, visualized with 3,3-diaminobenzidine (DAB kit, Vector Laboratories) followed by counterstaining with 10% Mayer's hematoxylin, and finally, mounted in Permamount (Vector Laboratories, Burlingame, CA), and examined by light microscopy.
The detection of the hybridization signal was with the Anti-DIG-FITC conjugate: counterstaining was with propidium iodide (PI) at a concentration of 2% in various antifade solutions.
Slides of formalin-fixed, paraffin embedded tumors were immunohistochemically stained involving rehydration, antigen retrieval, primary, antibody application, secondary antibody application, label application, staining, counterstaining.
Subsequently, the cells were stained using diaminobenzidine, restained with hematoxylin, and washed with running tap water for counterstaining.
Counterstaining of nuclei was performed with 2% Meyer's hematoxylin and mounted for examination.
01 M), incubated with secondary antibodies, and visualized with diaminobenzidine (DAB, brown color, ZSGB-BIO, Beijing, China) and hematoxylin counterstaining under a microscope.
After counterstaining with Hematoxylin for 1-2 min the slide is mounted in DPX.