This phenomenon could be reversed by C +A treatment (h, m), which was the most clear at 8 weeks pi (j, o), as shown by the determination of the immunostained caspase-3- positive cells and counterstaining
The use of combined FISH/GISH in conjunction with DAPI counterstaining
to identify chromosomes containing transgene inserts in amphidiploid tobacco.
(a) Anti-CD31 immunohistochemistry with USPIO particles counterstaining
shows blue iron particles within tumor vessels
(b) DAPI nuclear counterstaining
corresponding to (a).
Then, cells were incubated with DAPI counterstaining
solution for 5 min.
Hematoxylin was used for counterstaining
. The cells were identified based on the marker positivity and morphological features.
Immunofluorescence results revealed decreased [beta]-catenin immunofluorescence (green) in the cells treated with EGCG; DAP1 counterstaining
(blue) was similar in the cells treated with or not treated with EGCG (Fig.
Sections were then washed and incubated with donkey anti-mouse or anti-rabbit secondary antibodies prior to counterstaining
the nuclei with DAPI.
After a counterstaining
of nuclear with Hoechst 33342 (2 [micro]g/mL) for 5 min and wash, the ROS production was assessed using a fluorescence microscope (Olympus IX71) at 488/527 nm.
is almost always the blue hematoxylin coloring of nuclei.
This step was followed by detection using the Bond Polymer Refine kit for 25 min on the Bond Max Autostainer (Leica Biosystems, Newcastle upon Tyne, UK), visualization with diaminobenzidine chromogen, and counterstaining
In the laboratory, a counterstaining
technique using a rose Bengal (12.0 mg [L.sup.-1]) solution was applied to the sediment samples, and organisms identification was performed according to methods described in the literature [20-22].