The mean values (standard deviation (SD)) of the estimated sperm concentration as determined on the wet preparation, and the actual sperm concentration per Neubauer counting chamber
, are presented in Table 1.
In both laboratories, microscopic cell counts were performed by biomedical technicians using a Fuchs-Rosenthal counting chamber
(Assistent, Sondheim, Germany) after samples were stained with methyl violet acetic acid.
Then tow 35 ml samples were taken and diluted and the oocysts were counted microscopically using a McMaster counting chamber
. The total number of oocyst was calculated using the formula: Total oocyst = oocyst count (') dilution factor (') (fecal sample volume/counting chamber volume) .
Approximately 50 ml of the fecal- [K.sub.2][Cr.sub.2][O.sub.7] solution was retained to determine oocyst concentration later using a McMaster counting chamber
. The average oocyst count for each mouse in each dietary group each day after parasite inoculation was calculated using the following equation:
White cell count and red cell counts are directly estimated in the counting chamber
. However, it has been previously shown that results obtained by microscopy do not always agree with those from the Urisys 1800.
Viable cells were counted in the central reticule of a Neubauer counting chamber
using Trypan blue 1:9 (90 [micro]L of Trypan blue in 10 ?L of the sample).
The characteristics of boar sperm were analyzed for motility by light microscopy, viability by using a Makler counting chamber
and membrane integrity by a hypoosmotic swelling test (HOST).
Integral to the Auto T4 is the Cellometer disposable counting chamber
made of high-quality plastic materials.
1989) to digest the filter (2 h at 60[degrees]C), then washed twice in 0.2-[micro]m FSW, resuspended in 5 mL 0.2-[micro]m FSW, stained with Lugol's iodine and enumerated with a Hausser counting chamber
under 100x magnification.
In recent studies, the correlation (r) between UFC WBCs and WBCs by counting chamber
was 0.93-0.98, and that of UFC RBCs and RBCs by counting chamber
was 0.83-0.89 (10,25,26).
Cyanobacterial heterocysts were estimated using an inverted microscope and Sedgwick-Rafter counting chamber
In terms of WBC/hpt, a study by Alwall has documented very good correlation between strict quantitative counting chamber
methods and quick semiquantitative estimates of spun samples under a cover slip on a slide.