As raw polymer and fillers are identical in these recipes, it is reasonable to speculate that the rigidity of the samples is attributable to the crosslink
density of the vulcanized samples, as they have the same grade and dosage of polymer and filler in the recipes.
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The imposition of ultrasound further decreases the gel fraction and crosslink
density of the decrosslinked XHDPE with the effect increasing with the ultrasonic amplitude.
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The EDC and BDDGE treated tissues produced a characteristic cross-linking of the proteins which was observed in all crosslinked
samples, suggesting that the chemical treatment had effectively cross-linked the different chains of collagen proteins resulting into formation of high mass which did not find entry even in the stacking gel (Fig.
Hydroxyl radicals can further oxidize tyrosine, resulting in radical production and the formation of a bityrosine covalent crosslink
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To quantitatively indicate the effect of crosslinks
on [DELTA]SASA upon unfolding a new term called Crosslinking Factor (CLF) was introduced (CLF was described in Materials and Methods section.) Effectively, CLF is a measure of reduction in the SASA of unfolded protein as a consequence of presence of a single crosslink
, such as disulfide bond, and calculated to be equal to 918.5 [A.sup.2].
Dynamic mechanical analysis (DMA) of free films also is used to measure crosslink
densities of coatings.
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