The crude extract
obtained by EE presented fractions with the higher [iota]-CARs yields compared to those of RHWE and HWE, respectively (Table 2), confirming a previous study that used the papain digestion in the isolation of H.
Preparation of crude extracts
. The plant material (leaves) were collected at early morning hours and subjected to sun shade drying (15-20 days).
Experiments: Both the crude extract
and its three fractions were assessed for their feeding deterrence on H.
Broccoli extracts (chloroform, combined ethyl acetate and ethanol and the crude extract
) showed significant loss in body weight.
While the triterpene sapogenin (Oleszek 2002) content for the semi-purified extract was 72%, this decreased to 26% for the crude extract
. This can result from the type of extraction used, since this allows for the presence of different compounds from saponins such as sugars, among others (Guterres 2005).
Four to six animals in two experiments were included for each fraction or crude extract
The fibrinogenolytic activity was evaluated by incubating 20 [micro]g of human fibrinogen (Sigma Aldrich) with 5 [micro]g of the crude extract
for 15, 30, 45, 60 and 120 min.
The treatment with the crude extract
resulted in decrease of cell to cell surface attachment of P aeruginosa PAO1 with a hydrophobicity percentage of 29.19 [+ or -] 3.29% (Table 1).
Phytochemical analysis: The Caralluma edulis crude extract
was tested, for detection of various chemical constituents according to established protocols (Harborne, 1984; Evans, 1996).
Test for alkaloids: To 0.5 g of crude extract
, dilute hydrochloric acid was added and filtered.
Selection of these lipids was based on the preliminary miscibility studies with the crude extract
(data not shown).
Saline solution was used for the negative control mice, which had a body weight reduction by 0.7 while for test mice, 0.4 g weight was reduced in methanol crude extract
and 0.3 g was reduced in ethanol crude extract