Elliott, "Mesenchymal stromal cells derived from various tissues: biological, clinical and cryopreservation
aspects," Cryobiology, vol.
Some factors have direct impact on the risks of microbial pathogen contamination in gametes and embryos during cryopreservation
, such as the integrity of the embryonic zona pellucida (ZP), the freezing method, loading and sealing of the freezing container, and the sterility of both L[N.sub.2] and the storage container .
There are different cryopreservation
techniques, and the choice of which is most appropriate will depend on the explant to be cryopreserved (Sakai & Engelmann, 2007).
The science of plant cryopreservation
began with the freezing of mulberry twigs in LN in 1965.
has not yet been successfully applied to large structures, such as kidneys for transplantation.
Taurine conserved the concentration of superoxide dismutase and catalase during cryopreservation
. These enzymes scavenge the intracellular and extracellular superoxide anion and reduced the lipid peroxidation in plasma membrane (Perumalet al., 2013).
One problem with the procedure, however, is that no one knows the kind of effects ovarian tissue cryopreservation
will have on people as time goes by and it could take several decades before potential problems could be identified.
* The new polymers can reduce the amount of organic solvent required in cryopreservation
(freezing cells) as well as giving more and healthier cells after thawing
"Ultrarapid cooling is much faster than the cooling rate that is typically used in cryopreservation
. We call it superflash freezing, and it can almost vitrify and cryopreserve living cells without any cryoprotectant agent," said Yoshitake Akiyama.
Early referral to a multidisciplinary team offers selection of the most required fertility preservation method for fertility preservation, between the standard methods such as embryo or oocyte cryopreservation
and experimental options including ovarian tissue cryopreservation
of immature oocytes and ovarian suppression with gonadotropin-releasing hormone agonists (GnRHa) (19).
Critical vitrification steps towards survival of Garcinia hombroniana (Clusiaceae) shoot tips after cryopreservation