Yeasts were a very common isolate on culture media without cycloheximide
, their main representative being Candida spp.
The enhancing effect of luteolin at 5 [micro]M on ALP activity in MG-63 cells was blocked completely by the presence of cycloheximide
, an inhibitor of protein synthesis.
The sediment of specimens was inoculated on Sabouraud dextrose agar, Sabouraud dextrose agar (Merck, Germany) with cycloheximide
(Sigma-Aldrich, USA), paraffin agar (0.05 g FeS[O.sub.4], 0.05 g MgS[O.sub.4]x7[H.sub.2]O, 1 g [K.sub.2]HP[O.sub.4], 5 g N[H.sub.4]Cl, 0.05 g ZnS[O.sub.4], 1 g N[H.sub.4]N[O.sub.3], 0.05 g MnS[O.sub.4], 3 g K[H.sub.2]P[O.sub.4], 17 g Bacto Agar and 1 liter distilled water) and McClung's carbon-free broth tube (0.5 g MgS[O.sub.4]x7H, 2 mg ZnS[O.sub.4], 10 mg Fe[Cl.sub.3], 8 mg Mn[Cl.sub.2] x 4[H.sub.2]O, 0.8 g [K.sub.2]HP[O.sub.4], 2 g NaN[O.sub.3], 1 liter distilled water, pH 7.2) with placement of a paraffin coated glass rod.
To this end, we pretreated PBMCs with cycloheximide
(CHX), an inhibitor of translation.
Subsequently couplets were washed three times and cultured in PZM3 with 10 g/ml of CB and 10 g/ml of cycloheximide
. Four hours later the fusion results were examined under a stereomicroscope.
(1993) Differential action of cycloheximide
and activation stimuli on transcription of tumor necrosis factor-alpha, IL-1 beta, IL-8, and P53 genes in human monocytes.
At our institution, we use Sabouraud Dextrose, Brain Heart Infusion with 10% sheep blood with and without Chloramphenicol and Gentamicin, Inhibitory Mold Agar (Chloramphenicol), and Mycosel (Chloramphenicol and Cycloheximide
It is germ tube test negative, hydrolyzes urea, produces melanin, and does not grow on cycloheximide
Mycological analysis was performed by direct examination and culture in Sabouraud medium with chloramphenicol and cycloheximide
All media were supplemented with cycloheximide
(50 mg/L), nystatin (50 mg/L), and nalidixic acid (20 mg/L)  and incubated at 28[degrees]C for 1-4 weeks.
The animals of group I (treated group) were given cycloheximide
(CHM) solution (0.6mg/mL) at a dose of 3 mg/kg intraperitoneally (i.p.); group II (control group) was given equal volume of saline intraperitoneally (i.p.).
P Highfield, "Inhibition of DNA synthesis in synchronized Chinese hamster cells treated in G1 with cycloheximide
," Experimental Cell Research, vol.