Cytochemistry

(redirected from cytochemical)
Also found in: Dictionary, Medical.
Related to cytochemical: cytochemistry

cytochemistry

[¦sīd·ō′kem·ə·strē]
(cell and molecular biology)
The science concerned with the chemistry of cells and cell components, primarily with the location of chemical constituents and enzymes.

Cytochemistry

 

a branch of cytology that studies the chemistry of cell structures and the location of chemical compounds within a cell and their transformations in connection with the functioning of the cell and its individual components.

Cytochemistry arose in the 1820’s chiefly owing to the research of the French botanist F. V. Raspail, who summarized his ideas on cytochemistry in his work Essaie de chimie microscopique appliquée à la physiologie (1830). Staining techniques were subsequently developed to observe carbohydrates, proteins, amino acids, mineral compounds, and lipids under the microscope. The introduction of the use of aniline dyes in the late 19th and early 20th centuries led to major advances in cytochemistry. The principal approach in cytochemistry involves conducting appropriate chemical reactions in histological specimens and then evaluating them under a microscope. The evaluation may be qualitative (visual) or quantitative, using cytophotometry, autoradiography, and other methods.

The use of electron microscopy and immunochemical techniques in cytochemistry has been developing rapidly in recent years. Also used are microchemical methods, which make it possible to excise and examine individual cells, and centrifugation, which makes it possible to obtain tissue fractions abounding in certain types of cells or subcellular structures, such as nuclei, mitochondria, microsomes, and cytoplasmic membranes.

The main achievements of cytochemistry include the demonstration of the constant quantity of the DNA in the chromosome set, as well as the demonstration of the participation of macro-molecules (nucleic acids and proteins) in the specific functional activity of the cell and their migration within the cell from the nucleus to the cytoplasm and from the cell body to the outgrowths and back.

REFERENCES

Pearse, A. G. E. Gistokhimiia teoreticheskaia i prikladnaia. Moscow, 1962. (Translated from English.)
Vvedenie v kolichestvennuiu tsitokhimiiu. Moscow, 1969. (Translated from English.)

L. Z. PEVZNER

References in periodicals archive ?
Morphologic, cytochemical, and immunocytochemical studies confirmed the diagnosis of AML (M4-FAB).
Cytochemical Analysis of Laticifers and Meristematic Cells in the Shoot Apex of Milkweed
Cytochemical and ultrastructural evolution of orbicules in Lilium.
Tenders are invited for Supply of Non- specific Esterase Cytochemical kit(Sigma/Trinity/Merck) ,Specific choloroacetate Esterase (Sigma/Trinity /Merck) ,Myeloperoxidase staining kit.
In majority of the diagnostic centres in Pakistan, FAB classification is still widely used because it is based on morphology and cytochemical stains and does not require more advanced and costly equipment.
The cytochemical staining and measurement of protein with mercuric bromphenol blue.
The diagnosis of MPO deficiency was rare before 1979; currently, the diagnosis is easily made due to the widespread use of automated flow cytochemical analysis in clinical hematology laboratories for enumerating peripheral blood neutrophils with peroxidase activity (2).
Studies on fine structure and cytochemical properties of erythrophores in swordtail.
Cytochemical stains performed on bone marrow aspirate smears may also be useful when there is atypical nuclear morphology or cytoplasmic hypogranulation.
Cytochemical localization of peroxidase activity in the miracidium of Schistosoma mansoni.
Cytochemical demonstration of latency of lysosomal hydrolases in digestive cells of the common mussel, Mytilus edulis, and changes induced by thermal stress.