Also found in: Dictionary, Medical, Wikipedia.
Related to Cytochemistry: histochemistry


(cell and molecular biology)
The science concerned with the chemistry of cells and cell components, primarily with the location of chemical constituents and enzymes.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
The following article is from The Great Soviet Encyclopedia (1979). It might be outdated or ideologically biased.



a branch of cytology that studies the chemistry of cell structures and the location of chemical compounds within a cell and their transformations in connection with the functioning of the cell and its individual components.

Cytochemistry arose in the 1820’s chiefly owing to the research of the French botanist F. V. Raspail, who summarized his ideas on cytochemistry in his work Essaie de chimie microscopique appliquée à la physiologie (1830). Staining techniques were subsequently developed to observe carbohydrates, proteins, amino acids, mineral compounds, and lipids under the microscope. The introduction of the use of aniline dyes in the late 19th and early 20th centuries led to major advances in cytochemistry. The principal approach in cytochemistry involves conducting appropriate chemical reactions in histological specimens and then evaluating them under a microscope. The evaluation may be qualitative (visual) or quantitative, using cytophotometry, autoradiography, and other methods.

The use of electron microscopy and immunochemical techniques in cytochemistry has been developing rapidly in recent years. Also used are microchemical methods, which make it possible to excise and examine individual cells, and centrifugation, which makes it possible to obtain tissue fractions abounding in certain types of cells or subcellular structures, such as nuclei, mitochondria, microsomes, and cytoplasmic membranes.

The main achievements of cytochemistry include the demonstration of the constant quantity of the DNA in the chromosome set, as well as the demonstration of the participation of macro-molecules (nucleic acids and proteins) in the specific functional activity of the cell and their migration within the cell from the nucleus to the cytoplasm and from the cell body to the outgrowths and back.


Pearse, A. G. E. Gistokhimiia teoreticheskaia i prikladnaia. Moscow, 1962. (Translated from English.)
Vvedenie v kolichestvennuiu tsitokhimiiu. Moscow, 1969. (Translated from English.)


The Great Soviet Encyclopedia, 3rd Edition (1970-1979). © 2010 The Gale Group, Inc. All rights reserved.
References in periodicals archive ?
Distribution of acute cases of leukaemia as per cytochemistry. Out of 56 cases in total, 36 cases were acute, AML was 24, which was 67%, and ALL was 12, at 33%, for the cytochemical staining with both SBB, and PAS.
Flow cytometry with or without cytochemistry for the diagnosis of acute leukemias?
World Health Organization 2008/2016 Criteria for Mixed-Phenotype Blasts (a) Lineage Markers Myeloid MPO (flow cytometry, immunohistochemistry, or enzyme cytochemistry) -OR- Monocytic differentiation (at least 2 of the following: NSE cytochemistry, CD11c, CD14, CD64, lysozyme) T lineage Strong (b) cytoplasmic CD3 -OR- Surface CD3 B lineage Strong (b) CD19 with at least 1 of the following strongly expressed: CD79a, cytoplasmic CD22, or CD10 -OR- Weak CD19 with at least 2 of the following strongly expressed: CD79a, cytoplasmic CD22, or CD10 Abbreviations: MPO, myeloperoxidase, NSE, nonspecific esterase.
Kriz, "Podocytes in glomerulus of rat kidney express a characteristic 44 KD protein," Journal of Histochemistry and Cytochemistry, vol.
Chao, "Localization and expression of tissue kallikrein and kallistatin in human blood vessels," Journal of Histochemistry & Cytochemistry, vol.
Ploug, "Expression of C4.4A, a structural uPAR homolog, reflects squamous epithelial differentiation in the adult mouse and during embryogenesis," Journal of Histochemistry and Cytochemistry, vol.
Wang et al., "Therapeutic effects of suppressors of cytokine signaling in diabetic nephropathy," The Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society, vol.
The cytochemistry of both forms of M3 shows characteristic strong positivity for peroxidase or Sudan Black B and for chloroacetate esterase.
Hyphal interaction between Trichoderma harzianum, and Rhizoctonia solani: Ultrastructure and gold cytochemistry of the mycoparasitic process.
(University of Minnesota) and his collaborators reported their findings in An Innovative Method for Obtaining Consistent Images and Quantification of Histochemically Stained Specimens (Journal of Histochemistry and Cytochemistry, April 2015).