These reports suggest that shoot regeneration using purine based cytokinins
alone or in combination with auxins gave better results.
Letham and Palmi (1983) suggested that several factors could influence the results of cytokinin
As for the media supplemented with a single cytokinin
in combination with 0.5 [micro]M IBA and 50 [micro]M ADS, high level of callus formation was again observed for both the leaf blade and petiole explants and shoot bud formation was observed only in the medium containing BAP and TDZ.
Effect of cytokinins
. The excised cormlets were transferred aseptically in MS medium supplemented with different concentrations (1.00, 1.50, 2.00, 2.50, 3.00, 3.50, 4.00, 4.50 and 5.00 mg/l) of BAP for single shoot regeneration.
TABLE 3: Effects of different concentrations and combinations of cytokinin
and auxin on shoot production from two different sources of explants in yellow C.
1993, Kinet 1993), however, cytokinins
in combination with Auxins may promote in vitro flowering reported earlier (Naor et al.
The better cytokinin
was still BAP, which produced the highest proliferation rate (75%) at 0.6 [micro]M.
Increased seed cytokinin
levels in transgenic tobacco influence embryo and seeding development.
Thus far, three key hormones have been identified in these studies: abscisic acid and ethylene, which usher in dormancy; and cytokinins
, which stop it so sprouting can begin.
The hormones accumulating in infected plant cells were identified as [beta]-indoleacetic acid (IAA) in 1937, and a cell-division factor were later identified as a cytokinin
(Link et al., 1937).
Extracellular inverstase is an essential component of cytokinin
mediated delay of senescence.