Telophase enucleation: an improved method to prepare recipient cytoplasts
for use in bovine nuclear transfer.
The resulting pronuclei (karyoplasts) in the donor oocytes were microsurgically removed and discarded, and the pronuclei from the patient's oocytes were transferred into the donor's enucleated cells (cytoplasts
A 25 x 30 mm textured high-density polytetrafluoroethylene (d-PTFE) nonresorbable membrane (Cytoplast
GBR-200 singles, Osteogenics Biomedical, Lubbock, TX) was trimmed and placed under the buccal flap to replace completely the missing bony wall.
-fibroblast complexes were equilibrated with fusion medium consisting of 0.3 M mannitol solution containing 0.5 mM Hepes, 0.1 mM Ca[Cl.sub.2], and 0.1 mM Mg[Cl.sub.2].
Donor cells were fused with the recipient cytoplasts
with a single direct current pulse of 200 V/mm for 20 msec using an embryonic cell fusion system (ET3, Fujihira Industry Co.
Additionally, hepatocytes were extremely swollen, with condensed karyon and loosened cytoplasts
. Following the silymarin or PD treatments, the impaired cells were progressively recovered, inflammatory reaction were significantly inhibited, and necrotic and apoptotic mass were reduced (Figures 3(c)-3(f)).
Segal, "The bactericidal effects of the respiratory burst and the myeloperoxidase system isolated in neutrophil cytoplasts
," Biochimica et Biophysica Acta, vol.