Methods: TOL has a phenolic hydroxyl group that reacts with dansyl chloride.
Conclusion: A simple, cost-effective, and sensitive spectrofluorimetric method was developed and validated for the determination of TOL in human plasma and pharmaceutical preparations using dansyl chloride.
Keywords: Tolterodine, dansyl chloride, pharmaceutical preparations, plasma, derivatization
Development of a rapid and sensitive LC-ESI/MS/MS assay for the quantification of propofol using a simple off-line dansyl chloride derivatization reaction to enhance signal intensity J Pharm Biomed Anal 2005; 39: 411-7.
CO and 400 [micro]L of dansyl chloride
(5 mg per mL of acetone) at room temperature, and mixed for 90 min at 70 [degrees]C in the dark.
HPLC-grade acetonitrile and methylene chloride were obtained from EM Science, and dansyl chloride, estriol, estrone sulfate, and ethinylestradiol were obtained from Sigma-Aldrich.
Derivatization with dansyl chloride, as described below, was highly effective and was therefore used for all studies described for the LC-MS/MS method (31).
To derivatize the samples, we then added an equal volume of 1 g/L dansyl chloride in acetone, vortex-mixed each sample for 1 min, and incubated the samples in a heating block at 60[degrees]C for 3 min.
A sensitive fluorescence technique using dansyl chloride
to assess hair damage.
Gal-Hyl can be measured using an amino acid analyzer (12), but simpler methods have been developed for measurement of urinary Gal-Hyl, using HPLC after derivatization with dansyl chloride (13-16).
We used HPLC-grade acetonitrile (Rathburn Chemical), glass-distilled acetone (Rathburn), HPLC-grade isopropanol (Fluka), glacial acetic acid (BDH), anhydrous sodium acetate, 5-dimethylaminonaphthalene-l-sulfonyl chloride, dansyl chloride, sodium carbonate, and L-lysine (Sigma Chemical).
5 mol/L sodium carbonate solution in distilled water; 20 g/L dansyl chloride in acetone; 0.