As you may know, chitosan is a polysaccharide produced by the alkaline deacetylation
of chitin, a widely available biopolymer found in the exoskeleton of crustaceans such as crab and shrimp.
However, the direct causes of gene silencing are epigenetic modifications (18), as the methylation of cytokines in the gene promoter and deacetylation
of histone tails H3 and H4, among others (20) lead to absence of transcription of the gene and consequently the absence of FMRP (16).
Chitosan is a derivative of chitin which is obtained from chitin deacetylation
(Ngo et al, 2008; Azuma et al.
The levels of superoxide dismutase (Sod2) and Sirtuin 1 (Sirt1), a deacetylation
enzyme, were detected by Western blotting.
is required for the maturation of newly replicated chromatin.
Since it was first discovered in nuclear histones , the enzymes that catalyze protein acetylation and deacetylation
are subsequently referred to as histone acetyl-transferase (HAT) and deacetylase (HDAC).
Histone acetylation is catalyzed by histone acetyltransferases (HATs), whereas histone deacetylation
is catalyzed by HDACs.
The biopolymer used in this study is chitosan, which is obtained from the deacetylation
of chitin (Figure 1).
The development of optimized techniques from the preparation of shells, through chitin extraction to chitosan production aims to obtain chitosan with specific characteristics depending on the type of application, with emphasis on the degree of deacetylation
and the molar mass of the biopolymer (Yue, Yao, & Wei, 2009; Arantes et al.
Thus, elucidating alcohol-induced epigenetic changes opens new avenues for therapy of alcohol abuse and the resulting organ damage, such as the use of compounds that prevent histone deacetylation
Chitosan is a deacetylation
product of chitin, an abundant naturally occurring polysaccharide.
w] [greater than or equal to] 20,000 Da, degree of deacetylation
[greater than or equal to] 90%); triethylamine, Sulfanilic acid, Phenol, N-methyl pyrrolidone, and polyvinylpyrrolidone were analytical grade and purchased from Sinopharm Chemical Reagent; chloroacetic acid, acetic acid, hexanedioic acid, acetone, glutaraldehyde, and polyvinyl alcohol were obtained from Shenyang Xinxi reagent Factory; Cholesterol was purchased from Henan xiayi Bell biological Products and polysulfone was purchased from Shanghai Shuguang chemical plastics industrial corporation.