LPA is produced by fatty acid deacylation
of phosphatidic acid (PA) by [PLA.
found that the deacylation
activity of the mutation Q50[beta]M-Y149[alpha]K-F177[alpha]G toward CPC improved by 7.
Denu, "Activation of the protein deacetylase sirt6 by long-chain fatty acids and widespread deacylation
by mammalian sirtuins," The Journal of Biological Chemistry, vol.
The first stage occurring at the range of 300-400[degrees]C is related to the expansion of EG and the deacylation
of the EVA phase through radical and ionic [beta]-elimination mechanism [34, 35].
Although it is known that the acylation step is rate-determining ibr simple amide substrates (Brouwer and Kirsch, 1982; Ayala and Di Cera, 2000) like BApNA, deacylation
can be rate-limiting for peptide substrates of lobster trypsins as has been reported for their vertebrate counterparts (Hedstrom et al.
4) Young and Buchanan (6) partially solved the problem by deacylation
of PGL-I, i.
The transcriptional suppression occurs because deacylation
of histone protein creates conformational changes, limiting access and binding of transcription factors and RNA polymerase to related genes (Figure 3A) .
We optimized this deacylation
procedure and quantified the sphingoid bases and lysoglycosphingolipids after derivatization with ophthaldialdehyde (OPA) by HPLC with fluorometric detection (24).
4-PpPbp (S649F and N651I) plasmid had two mutation sites in motif 7, which is thought to function in the correct positioning of substrates or contribute to deacylation
activity, (17) while two mutations were inserted into the non-conserved amino-acid residues between motifs 6 and 7 of the TP domain in the pTFH22.
The first stage occurring between 300 and 400[degrees]C was related to the deacylation
of the EVA phase through radical and ionic [beta]-elimination mechanism [20, 21], The second stage which took place in the temperature range of 400-530[degrees]C involved the degradation of the HDPE phase accompanied with the decomposition of the polyacetylene-ethylene chains formed in the first stage .
notatum enzyme catalyzed rapid deacylation
of purified ovolecithin in the absence of lipid activators such as cardiolipin, provided if the substrate was dispersed in a sufficiently fine state.
1998) Mammalian glycophosphatidylinositol anchor transfer to proteins and post-transfer deacylation