(6) Time and water saving acid removal from tooth: in most of the previous studies the acid removal from the tooth is done under the flow of running water for 24 or more hours (Culling 1957), which may be because in these studies the complete tooth was
decalcified and it took more time to completely remove the acids from it.
After overnight fixation at 4[degrees]C, the temporal bones were
decalcified with 0.12 M EDTA in PBS for 7 days at 4[degrees]C.
The cochleae were removed from anesthetized mice, fixed in 4% formaldehyde in 10 mM PBS at 4[degrees]C overnight, and
decalcified in 10% EDTA at room temperature for 2 days [24, 26].
The tissue sample was immersed in formalin solution for 24 h for fixation and subsequently
decalcified. The interface between the tumor and normal bone marrow was grossly determined and submitted for microscopic evaluation.
Unfortunately, analogous data are not available for
decalcified tissues because questions regarding validation practices on
decalcified tissues were not included in the 2010 questionnaire; however, new benchmark data are now available.
The
decalcified bone was cut into transversal sections and photodocumented with a magnification factor of 22.
The histopathologic sample was fixed in 10% buffered formalin and subsequently
decalcified in 10% formic acid.
And then, the dehydrated samples were
decalcified using decalcifying solution-Lite (Sigma-Aldrich, St.
It is recommended to check sections after 5-10 min and re-immerse in RDO if not adequately
decalcified. In ge neral, larger vertebra sections require longer soak times, but a total soak time of roughly 15 min is adequate for most specimens.
The samples were fixed with neutral buffered formalin solution and
decalcified with 5 % EDTA (Ethylenediaminetetraacetic acid).