decolorizer


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decolorizer

[dē′kəl·ə‚rīz·ər]
(chemical engineering)
An agent used to decolorize; the removal of color may occur by a chemical reaction or a physical reaction.
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The decolorizer should flow over the stained area of the slide (not be applied directly) until all color has washed out.
* Apply decolorizer so it runs over the stained area until no more color washes out.
* Rinse gently under running water and allow the slide to air dry Modifications to Gram's stain and their applications Modification Application Addition of ammonium oxalate Prevents precipitation of dye to crystal violet Substitution of polyvinylpyrrolidone Iodine remains stable in solution iodine for Gram's iodine Variation in decolorizer: --Acetone --Rapid decolorization --Acetone-alcohol --Slower decolorization --Alcohol --Slowest decolorization Substitution of carbol fuchsin Improved demonstration of or 0.8% basic fuchsin for safranin weakly staining Gram negatives Addition of fast green and Improved contrast between tartrazine before counter- Gram negatives and back- staining with safranin ground
The positive result for the presence of AFB was confirmed using ZN technique which includes strong carbol fuschin as primary stain, 20% sulphuric acid as decolorizer and 0.1% methylene blue as counter stain (7).
Therefore, production of some exopolysaccharides or capsular elements by the best decolorizers could be actual reason for adsorption of color from the wastewater.