This golden standard that all corporate players need to adhere to, even reluctantly, ignorantly or in contradiction to their authentic beliefs it relies on the savior's complex, demasking
an act of captatio benevolentiae in disguise.
Samples were exposed to citrate buffer to allow demasking
of epitopes and were permeabilized with 1% Triton X-100 (Sigma-Aldrich) in Dulbecco's phosphate buffered saline (DPBS) (Sigma-Aldrich) for 10 min.
As such, the demasking
protocol will need to be optimized for each drug and may require a combination of strategies.
Immunohistochemical studies were performed with pre-treatment demasking
in a microwave oven and using a Novostain Universal Detection Kit detection system (Novocastra Laboratories Ltd., UK).
All slides were warmed up for 35 min to a temperature of 55[degrees]C for dewaxing, boiled in 10 mM citrate buffer (pH 6.0) by microwave treatment (600 W) for 20 min for demasking
Briefly, after deparaffinizing and demasking
of antigens, the slides were then soaked in 90 mL of methanol/10 mL 30% [H.sub.2][O.sub.2] for 10-15 min at room temperature to block endogenous peroxidases and then washed with PBS.
Acknowledging the implications of the public secrecy analytic, Zizek again echoes Taussig in cautioning that "we must avoid the simple metaphors of demasking
, of throwing away the veils which are supposed to hide the naked reality.
(2010) have established the appropriateness of the account localist models offer for the processing of cognates and semi-cognates on the basis of the lexical decision task, language decision task, and the progressive demasking
task (8) with cognates.
After antigen demasking
by microwav-ing sections in citrate buffer (10 mmol [1.sup.-1], pH 6.0), nonspecific background staining was blocked by section pre-incubation in phosphate-buffered saline containing 0.5% bovine serum albumin (Sigma, St.
Scenes of unmasking do not compromise the mask's ability to signify, but are instead integral to it, because "demasking
heightens the masking power of the mask" ("Face" 232).
Os pesquisadores se basearam no paradigma do desmascaramento progressivo (progressive demasking
task) e realizaram sua pesquisa com estudantes universitarios falantes do Frances.
(3) This same metaphor is used by Basil later in the play while demasking
Moustique who is passing off as Makak in order to cash in on Makak's fame.