With the use of constant denaturing
capillary electrophoresis, formation of HDs was demonstrated and a simple "reconditioning PCR" suggested (Jensen 1993; Thompson & Marcelino 2002) to reduce the number of HDs produced.
PCR conditions for pap31 gene amplification were a single hot-start cycle at 95[degrees]C for 5 min, followed by 45 cycles of denaturing
at 94[degrees]C for 45 s, annealing at 58[degrees]C for 45 s, and extension at 72[degrees]C for 45 s.
Indeed, if we allow that small business can be specific in nature, we must also allow for the corollary of this statement, namely denaturing
Hetero-duplex formation was obtained by mixing together, denaturing
, and gradually reannealing equimolar quantities of PCR products for patients and controls.
Profiling of complex microbial populations by denaturing
gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA.
The nondenaturing electrophoresis system has nearly the same resolution as that obtained with denaturing
acrylamide gels and silver staining.
KEY WORDS: exclusive PCR; denaturing
gradient gel electrophoresis: cry4 gene; Bacillus thuringiensis.
But their underlying structure is linear, and that makes them susceptible to unfurling, or denaturing
EPIEN's products employ a patented chemical denaturing
system to more effectively cleanse wound tissue of bacteria and cell debris while reducing the patient's pain and inflammation.
The use ofbiocompatible options brings a streamlined approach to denaturing
DNA and semi-preparative proteomics separations.
Mutation detection sensitivity obtained with endoVII digestion was found to be similar to that for denaturing
HPLC and direct sequencing (13).
polyacrylamide gel materials were obtained from National Diagnostics (Atlanta, GA).