In performing dextran sedimentation, every 6 mL of blood or PBMC-depleted neutrophil/erythrocyte suspension (if preceding or following low-density Ficoll-Hypaque density gradient centrifugation, resp.) was mixed with 4 mL of 5% (w/v) dextran (MW 266000, Sigma-Aldrich) in 0.9% NaCl .
Neutrophils Purified by Dextran Sedimentation Followed by Ficoll-Hypaque Density Gradient Centrifugation Are Activated.
Density gradient centrifugation
compromises bone marrow mononuclear cell yield.
The viabilities of the fresh hemocytes collected from the oysters and the separated hemocyte subpopulations by Percoll discontinuous density gradient centrifugation
were estimated by the 0.1% (w/v) Trypan blue test.
Concurrently, free particulates were separated from T-cells by density gradient centrifugation
through Ficoll-Hypaque (Histopaque, Sigma, St.
Leukocytes remained in all four samples after simple centrifugation and washing and in two of three samples tested after density gradient centrifugation
. In two, CD4+ lymphocytes were present after simple centrifugation and washing.
A variety of microfluidics and filtration devices have been developed for the isolation of CTCs on the basis of the different properties of CTCs that distinguish them from the surrounding normal hematopoietic cells: (a) physical properties like size, density, electric charges, or deformability; (b) biological properties such as cell surface protein expression, which involve immunomagnetic bead separation with positive or negative selection; or (c) a combination of both with filtration-based size separation, antigen cell sorting using flow cytometry, and density gradient centrifugation
Both swim-up and density gradient centrifugation
methods have been evaluated for DNA damage.
Purification of large quantities of influenza virus by density gradient centrifugation
. J Virol 1967;1:1207-6.
In addition, the distribution of LDL subclasses was validated against an established method, discontinuous density gradient centrifugation
, on a KBr gradient as described by Griffin et al.
Mononuclear leukocytes were first separated by conventional density gradient centrifugation
or equivalent methods.