Thymidine

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thymidine

[′thī·mə‚dēn]
(biochemistry)
C10H14N2O5 A nucleoside derived from deoxyribonucleic acid; essential growth factor for certain microorganisms in mediums lacking vitamin B12 and folic acid.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
The following article is from The Great Soviet Encyclopedia (1979). It might be outdated or ideologically biased.

Thymidine

 

a natural organic compound, a nucleoside consisting of the pyrimidine base thymine and the carbohydrate deoxyri-bose. Thymidine is made up of acicular crystals that are readily soluble in water. With respect to chemical properties, it is a weak base. Thymidine is present in all living cells as a constituent of deoxyribonucleic acid (DNA). Thymidine tagged with radioactive tritium is extensively used in laboratories to study DNA biosynthesis and the repair of DNA after injury.

The Great Soviet Encyclopedia, 3rd Edition (1970-1979). © 2010 The Gale Group, Inc. All rights reserved.
References in periodicals archive ?
(9) Reverse transcriptase inhibitors (ex: 3' azido 3' deoxythymidine) (10) and natural products (ex.tclomestatin and sulfoquinovosyldiacyl glycerol) (11).
Thymidylate synthase (TS) is a 36-kDa dimeric protein that catalyzes methylation of deoxyuridine monophosphate (BUMP) to deoxythymidine monophosphate (dTMP), using the reduced folate 5,10-methylenetetrahydrofolate as the methyl donor (53, 54).
The reaction mixture contained 50 mmol KCl; 10 mmol Tris HCl, pH 8.5; 3 mmol Mg[Cl.sub.2]; 0.1% Triton X-100 (Nacalai Tesque, Kyoto, Japan); 2% formamide; 10 pmol of primer pairs; 200 [micro]mol each of deoxyadenosine triphosphate, deoxyguanosine triphosphate, deoxycytidine triphosphate, and deoxythymidine triphosphate; and 2.5 units recombinant Taq polymerase; the mixture then was overlaid with 25 [micro]L mineral oil.
Therefore, T vectors possessing two 3' overhanging deoxythymidines have been created using different approaches, either by tailing a single deoxythymidine to the 3' end of linear and blunt ended plasmids (2) or by cutting a modified plasmid with selected restriction enzymes such as Xcm I, to generate linear plasmids with single overhang deoxythymidine at both 3' ends (3-8).
The amplification solution consisted of the following: 1 [micro]mol/L of each consensus primer; 200 [micro]mol/L each of deoxyadenosine triphosphate, deoxycytidine triphosphate, and deoxyguanosine triphosphate; and 140 [micro]mol/L of deoxythymidine triphosphate.
The hydrolyzed samples were then injected into the HPLC and the 5-methyldeoxycytidine and deoxythymidine fractions were collected.
Radioactivity of methyldeoxycytidine and deoxythymidine was measured more specifically after DNA hydrolysis and HPLC analysis (Fig.
Methyldeoxycytidine and deoxythymidine were well separated.