In organisms that synthesize lysine, it has two main biosynthetic pathways, the diaminopimelate
, and aminoadipate pathways.
(1984) reported that Corynebacterium glutamicum and other glutamate producing bacteria possess diaminopimelate
dehydrogenase (DDH), which form meso- diaminopimelate
(meso-DAP) directly to form L- lysine.
In vitro transcription of linearized pBluescript KS + plasmids (Stratagene/Agilent Technologies) containing the coding sequences of Bacillus subtilis control genes lysA (diaminopimelate
decarboxylase) and pheB (phenylalanine biosynthesis associated protein) or the Arabidopsis thaliana control gene XCP2 (xylem cysteine peptidase 2) were performed with the T7 MEGAscript High Yield Transcription Kit (Ambion/ Applied Biosystems) according to the manufacturer's instructions.
The following were chosen from the small chromosome: dtdS, the encoding threonine dehydrogenase; lysA, the encoding diaminopimelate
decarboxylase; pntA, the encoding transhydrogenase alpha sub-unit; pyrC, the encoding dihydroorotase; and tnaA, the encoding tryptophanase.
du Pont de Nemours and Company (Wilmington, DE) has patented an isolated nucleic acid fragment encoding a plant enzyme that catalyze steps in the biosynthesis of lysine, threonine, methionine, cysteine and isoleucine from aspartate, the enzyme a member selected from the group consisting of: dihydrodipicolinate reductase, diaminopimelate
epimerase, threonine synthase, threonine deaminase and S-adenosylmethionine synthetase.
(1986) reported that neither N-hydroxy nor N-amino diaminopimelates
inhibited lysine decarboxylase from B.