Therefore we used the automated Sanger Dideoxy method
to control plasmid gWizHBs nucleotide sequence with focus on the sequence encoding for the hepatitis B s antigen.
The sequencing was done by the Sanger dideoxy method
(14) and this was followed by the detection of the HCV sequence on Beckman Coulter CEQ8000 genetic analysis system (Fullerton, USA).
Three clones were sequenced 3 times in both orientations by the dideoxy method
, using M13 universal primers and ET Dynamic Terminator for MegaBACE (GE Healthcare, Fairfield, CT, USA).
The Patent covers both the standard dideoxy method
used in shotgun sequencing as well as hybridization-based methods employed by biochips.
Nucleotide sequencing was performed by the dideoxy method