diethyl pyrocarbonate


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diethyl pyrocarbonate

[‚dī′eth·əl ‚pī·rō′kär·bə‚nāt]
(organic chemistry)
C6H10O5 A viscous liquid, soluble in alcohols, esters, and ketones; used as a gentle esterifying agent, as a preservative for fruit juices, soft drinks, and wines, and as an inhibitor for ribonuclease. Abbreviated DEPC.
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An advantage of this purification technique is that it eliminates the need to use diethyl pyrocarbonate (DEPC), a toxic compound that is expensive and time consuming to use.
Amplification was done in a 50-[micro]L reaction volume containing 10 [micro]L 5x buffer, 2 [micro]L deoxynucleotide triphosphate mix (10 mmol/L), 2 [micro]L enzyme mix (all from QIAGEN), 2 [micro]L RNase inhibitor (Promega, Mannheim, Germany), 1.25 [micro]L primers Pp1 and Pml (20 pmol/[micro]L; Jena-Bioscience, Jena, Germany), 27.5 [micro]L diethyl pyrocarbonate (DEPC)--treated water, and 4 [micro]L RNA extract.
Until recently the only method of achieving nuclease free water was with the use of DEPC (Diethyl pyrocarbonate), a chemical that will inactivate RNase.