Besides total protein recovery, differential extraction
of specific protein classes can occur as a function of the buffer composition and the processing of the food.
This method, originally applied to the process of differential extraction
of male/female DNA from a mixed forensic sample, has broad application in a wide variety of biological sample processes for analysis and identification.
This procedure is known as differential extraction (Gill et al.
At the same time, a robotic liquid handler will be programmed to execute a proprietary differential extraction procedure that is based on differential lysis and selective filtration.
The more we use the unit and explain its operation to our clients--and show them the "before" and "after" comparisons--the greater is our level of comfort in setting reasonable standards for how-clean-is-clean, Correlation of the ATP readings with CFUs has been attempted by various researchers, but it must be re-evaluated with each circumstance and is a complicated procedure involving filtration of the samples and differential extraction
of somatic ATP and microbial ATP.
Currently, the laboratory procedure to perform this tedious DNA processing technique, referred to differential extraction
, involves numerous laboratory-based, labor-intensive techniques that are subject to contamination and human error.
In Case 2, using the differential extraction protocol, stains N1, N2, and N3 gave no autosomal STR or Y-STR results.
The fact that no sperm cell was visibly detected from the Christmas Tree Staining Test and that the results from the differential extraction protocol were negative, the Y-STR results obtained from stains N1 and N2 could have originated from underlying traces of his epithelial, saliva, or other cells.