Quercetin, [beta]-glucuronidase (HP-2), Ethylenediaminetetraacetic acid (EDTA), acetylcarnitine, leupeptin, bovine serum albumin (BSA), phenylmethylsulfonylfluoride (PMSF), Tris, dimethyl sulfoxide
(DMSO), dithiothreitol (DTT), and carnitine acetyltransferase, Acetylcoenzyme, 2-Aminofluorene (2-AF) and N-acetyl-2-aminofluorene (2-AAF) were obtained from Sigma Chemical Co.
The major differences are the use of a lower MgCl2 concentration (0.625 mmol/L) and the use of dimethyl sulfoxide
(DMSO) (12%) instead of formamide.
HPLC-grade dimethyl sulfoxide
and HCl were obtained from Aldrich.
If this PCR is performed with reaction mixtures without dimethyl sulfoxide
, the resulting melting points will be ~3[degrees]C higher.
Louis, MO, USA) and TCHQ (Sigma Chemical Co.) with dimethyl sulfoxide
We prepared stock solutions of F[B.sub.1], DEN, and CAT by dissolving 0.5 mg of each toxin in 30 [micro]L ethanol, 170 [micro]L dimethyl sulfoxide
(DMSO), and 4.8 mL complete culture medium (CCM; minimum essential medium supplemented with 5% fetal calf serum, 1% Penstrep fungizone, and 1% L-glutamine), thus yielding a toxin concentration of 100 [micro]M.
(1), 1 [micro]L of reaction buffer (LightCycler DNA master hybridization probes 10 X buffer; Roche Diagnostics), 0.4 [micro]L of 25 mM MgCl, stock solution, 0.5 [micro]L of dimethyl sulfoxide
, and 0.1 [micro]M each of the probes.
PENNSAID is the only FDA-approved topical NSAID in a vehicle solution containing dimethyl sulfoxide
(DMSO), a known penetrating agent.
Peptides were resuspended in dimethyl sulfoxide
The PCR mixture contained 67 mM Tris-HCl (pH 8.4), 17 mM [(N[H.sub.4]).sub.2] S[O.sub.4], 6.7 mM Mg[Cl.sub.2], 0.1 g/L gelatin, 1 g/L Triton X-100, 0.4 [micro]M F6/F4, 80 [micro]M PCR-grade dNTPs (Roche Molecular Biochemicals), 2 [micro]L of dimethyl sulfoxide
(Merck), and 1 [micro]L of thawed cell lysate.
After incubation for 1 hr at 37[degrees]C, the plates were washed again, and an enzyme substrate solution of 3,3',5,5'-tetramethyl-benzidine (TMB; Sigma Chemicals; 100 [micro]L/well; 6 mg/mL dimethyl sulfoxide
) was added.
The drug combines the transdermal carrier (containing dimethyl sulfoxide
, DMSO) with 1.5% diclofenac sodium, an NSAID and delivers the active drug through the skin directly to the site of inflammation and pain, concluded the company.