stain

(redirected from double stain)
Also found in: Dictionary, Thesaurus, Medical.

stain

a dye or similar reagent, used to colour specimens for microscopic study

Stain (microbiology)

Any colored, organic compound, usually called dye, used to stain tissues, cells, cell components, or cell contents. The dye may be natural or synthetic. The object stained is called the substrate. The small size and transparency of microorganisms make them difficult to see even with the aid of a high-power microscope. Staining facilitates the observation of a substrate by introducing differences in optical density or in light absorption between the substrate and its surround or between different parts of the same substrate. In electron microscopy, and sometimes in light microscopy (as in the silver impregnation technique of staining flagella or capsules), staining is accomplished by depositing on the substrate ultraphotoscopic particles of a metal such as chromium or gold (the so-called shadowing process); or staining is done by treating the substrate with solutions of metallic compounds such as uranyl acetate or phosphotungstic acid. Stains may be classified according to their molecular structure. They may also be classified according to their chemical behavior into acid, basic, neutral, and indifferent. This classification is of more practical value to the biologist. See Medical bacteriology

Stain

A coloring liquid or dye for application to any porous material, most often wood; thinner than paint and readily absorbed by the wood so that the texture and grain of the wood is enhanced, and not concealed.

stain

[stān]
(materials)
A nonprotective coloring matter used on wood surfaces; imparts color without obscuring the wood grains.
Any colored, organic compound used to stain tissues, cells, cell components, cell contents, or other biological substrates for microscopic examination.

stain

1. A discoloration in the surface of wood, plastic, sealant, etc.
2. A colorant for enhancing wood grain during finishing.
References in periodicals archive ?
Few studies have investigated the value of Ki67 indices quantified by DIA on single IHC stains, and to our knowledge none have been performed on IHC double stains. In contrast to our study, studies on both breast cancer (19) and Hodgkin lymphoma (18) found manual and digital indices in close correlation (r = 0.98).
(13,15,17) Because Ki67/MART1 double stains visualize the actual tumor cells, we were unable to make such an index estimation on our double stains.
The authors thank the medical laboratory technicians Allan Thorsteinsson, Martin Nielsen, Lone Nielsen, and Helle Johnsen from the Department of Pathology, Vejle Hospital, for performing the immunohistochemical double stains. Visiopharm A/S did not provide financial support to this study.
Immunohistochemical double stains against Ki67/MART1 and HMB45/MITF: promising diagnostic tools in melanocytic lesions.
A newly developed double stain using MUC4 and p53 was then evaluated because these 2 antibodies show staining limited to the cytoplasm and nucleus, respectively, avoiding interference of interpretation between the stains.
When the novel MUC4/p53 double stain was used and a positive for either stain was considered a positive result, the sensitivity was 96% and the specificity was 73%.
The rationale for developing the double stain in this study was that there is not always sufficient tissue available for multiple stains when performed on different slides.
The sensitivity and specificity achieved by using MUC4/p53 double stain (96% sensitivity and 73% specificity) compares favorably with most other combinations and offers the advantage of evaluating the same gland on a single slide with 2 stains.