The conventional biochemical tests were: GS = Gram staining; OX = Oxidase; Mc = MacConkey medium; CI = Citrate; SIM = SIM medium; AR = Arabinose; CE = Cellobiose; RA = Raffinose; XY = Xylose; VP = Voges-Proskauer; LAC = Lactose; OR = Ornithine; DU = Dulcitol
perfringens biochemically was saccharolytic because it formed acid when it utilized glucose, sucrose, maltose and lactose but did not utilized mannitol and dulcitol
. The gas of fermentation was hydrogen sulphide.
Moreover, they fermented glucose, fructose, mannose, mannitol, sucrose, sorbitol and xylose without gas production but not ferment arabinose, inositol, lactose, maltose, salicin, dulcitol
Upon further testing with sorbitol and dulcitol
fermentation, the strain was found to belong to multocida subspecies.
auris from surveillance samples, we placed dulcitol
, instead of dextrose, in the selective enrichment media.
In her notebook, Inber described decadent plates of caviar and cheese sandwiches, whereas the publication describes a modest meal: "coffee with dulcitol
(something like saccharin but tastier) and slices of black bread, sprinkled with glucose.
Biochemical characterization of strain B14 Characteristic Strain B14 Colony pigmentation Creamy Motility Utilization of: + Lactose + Xylose + Maltose + Fructose + Dextrose + Galactose + Raffinose + Trihalose + Melobiose + Sucrose + L-arabinose + Mannose - Inulin + Sodium gluconate + Glycerol + Salicin - Dulcitol
- Inositol - Sorbitol - Mannitol + Adonitol - Arabitol - Erythritol - a-methyl-D-glucoside - Rhamnose - Cellobiose + Melezitose + a-methyl-D-mannoside - Xylitol - D-arabinose + Citrate - Malonate - Sorbose - ONPG hydrolysis + Esculin hydrolysis + Table 2.
None of strains fermented rhamnose, lactose, trehalose, raffinose, dulcitol
Haplotypes were tested by API 50CHB strips (BioMerieux, Inc., France) for utilization of the following substrates: glycerol, erythritol, D-arabinose, L-arabinose, ribose, D-xylose, L-xylose, Adonitol, B-methyl xyloside, galactose, glucose, fructose, mannose, L-sorbose, rhamnose, dulcitol
, inositol, sorbitol, mannitol, L-methyl-D-mannoside, D-methyl-D-glucoside, N-acetylglucosamine, amygdalin, arbutin, aesculin, salicin, cellobiose, maltose, lactose, sucrose, Trehalose, gentiobiose, melibiose, raffinose, melezitose, starch, glycogen, inulin, D-turanose, D-tagatose, D-fucose, L-fucose, D-lyxose, D-arabitol, L-arabitol, xylitol, gluconate, and 2,5-ketogluconate.
It is seen as small, circular, raised, whitish with black center on XLD and pale on MacConkey agar, grow best at a temperature between 25[degrees]C-37[degrees]C, PH 7-8 and 0.5%NaCl [7,8] and characteristically, catalase positive, cytochrome oxidase negative, glucose fermentative, indole positive, citrate negative, lysine positive, mannitol, dulcitol
, sorbitol, inositol, xylose, rhamnose negative, produce hydrogen sulfide, alkaline slant and acid but on Triple sugar iron Agar .
multocida can be determined by sorbitol and dulcitol
fermentation, though this is rarely performed.
One or two drops of the culture suspension from the peptone were transferred to the 5 tubes containing glucose, lactose, sucrose, mannitol and dulcitol
. The reactions were noted for acid formation or acid and gas formation.