Linearized targeting construct (10 [micro]g, TakaRa, Shiga, Japan) and Each TALEN expression plasmid (15[micro]g) were electroporated
to passage one cells (5x[10.
Screening the electroporated
cells was done for antibiotic resistance and performed on nutrient agar plates supplemented with 5 and 10 mg/ml of neomycin '4.
tuberculosis H37Rv, except for pPr26 that was electroporated
only into M.
As in lamprey, regenerative capacity can also be modulated in the larval zebrafish: Mauthner axons demonstrate minimal regeneration after spinal cord damage (Bhatt et al, 2004), but when cAMP is electroporated
into the soma, axons sprout and functional regeneration occurs (Bhatt et al, 2004.
5]) were electroporated
using Amaxa Nucleofector (Lonza, Basel, Switzerland) in 100 [micro]L electroporation buffer [20 mM HEPES, pH 7.
Transient gene expression in electroporated
banana (Musa spp.
Cells were also electroporated
with only plasmid DNA (pSD5) as expression control.
Failure to immediately added SOC to the electroporated
cells can significantly reduce cell viability and decrease transformation efficiency.
Transient gene expression in secondary somatic embryos from coffee tissues electroporated
with the gene gus and bar.
Development of a mass gene transfer method in Chinook salmon: optimization of gene transfer by electroporated
cells were resuspended in 980 [micro]L SOC medium (Sambrook et al.
After insertion the electroporated
cells were plated and allowed to grow.