electroporation

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electroporation

[i‚lek·trō·pə′rā·shən]
(biology)
The application of electric pulses to increase the permeability of cell membranes.
(cell and molecular biology)
The application of electric pulses to animal cells or plant protoplasts to increase membrane permeability.
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Linearized targeting construct (10 [micro]g, TakaRa, Shiga, Japan) and Each TALEN expression plasmid (15[micro]g) were electroporated to passage one cells (5x[10.
Screening the electroporated cells was done for antibiotic resistance and performed on nutrient agar plates supplemented with 5 and 10 mg/ml of neomycin '4.
As in lamprey, regenerative capacity can also be modulated in the larval zebrafish: Mauthner axons demonstrate minimal regeneration after spinal cord damage (Bhatt et al, 2004), but when cAMP is electroporated into the soma, axons sprout and functional regeneration occurs (Bhatt et al, 2004.
5]) were electroporated using Amaxa Nucleofector (Lonza, Basel, Switzerland) in 100 [micro]L electroporation buffer [20 mM HEPES, pH 7.
Cells were also electroporated with only plasmid DNA (pSD5) as expression control.
Failure to immediately added SOC to the electroporated cells can significantly reduce cell viability and decrease transformation efficiency.
Transient gene expression in secondary somatic embryos from coffee tissues electroporated with the gene gus and bar.
Development of a mass gene transfer method in Chinook salmon: optimization of gene transfer by electroporated sperm.
Electroporated cells were resuspended in 980 [micro]L SOC medium (Sambrook et al.
After insertion the electroporated cells were plated and allowed to grow.