LC-MS/MS revealed that DBP and DEHP entered the cells, where they were converted to monoesters, whereas the monoesters MBP and MEHP were excluded probably because of their negative charge (Figure 2F)-Because MBP has been argued to be the active metabolite of DBP, we electroporated
10 [micro]M and 100 [micro]M MBP into the SC5 cells, which at 100 [micro]M resulted in a significant inhibition of PGD2 after 6 hr (Figure 2G).
7] per electroporated
cells (Denning and Priddle, 2003).
In addition, this study showed that electroporated
tumors contained CD4+ and CD8+ lymphocytic infiltrates in the treated lesions, further validating the ability of DNA IL-12 to up-regulate adaptive and innate immunity.
Partial correction of the single-strand break repair defect in the CHO mutant EM9 by electroporated
recombinant XRCC1 protein.
Virus recovered from baby hamster kidney cells electroporated
with transcribed RNA was used for all experiments.
Cells were electroporated
to deliver labeled peptides into the cytoplasm.
coil cells were electroporated
with the pMAL c2x expression vectors and then microgram amounts of plasmid DNA BamH1 V,6H pMAL c2x vectors were purified.
As a first step, a DNA construct encoding Green Fluorescent Protein (GFP) was injected into the lumen of the neural tube and electroporated
into embryos at two days of development (Stage 14).
These responses occurred over 6 - 18 months, with gradual volume loss occurring at sites distinct from the electroporated
sites, arguing for immune system involvement.
6] cells) were electroporated
using a 100 [micro]L Nucleocuvette, CA137 program code, in an Amaxa 4D-Nucleofector (Lonza, P3 Primary Cell 4D-Nucleofector X Kit L) with a total of 45 [micro]g plasmid DNA at a 2:2:1 weight ratio (plasmid encoding a left ZFN: plasmid encoding a right ZFN: eGFP reporter or H-2[K.
The study was based on a mouse model of type 1 diabetes that shares many similarities with human models to demonstrate the therapeutic effectiveness of dendritic cells electroporated
(eDC) with translationally enhanced interleukin-4 (IL-4) messenger RNA, known as eDC/IL-4.
cells (cells with DNA physically introduced) were mixed with SOC medium (12) and incubated for 60 minutes at 37[degrees]C before plating on LB with ampicillin plates.