electroporation


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electroporation

[i‚lek·trō·pə′rā·shən]
(biology)
The application of electric pulses to increase the permeability of cell membranes.
(cell and molecular biology)
The application of electric pulses to animal cells or plant protoplasts to increase membrane permeability.
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References in periodicals archive ?
Available Samples: Mirus/MoBiTec consider sample requests for up to three different transfection products and one electroporation product per laboratory.
TRACE technology incorporates an electrochemical tissue-sensing control system to automatically adjust pulse width and treatment duration in real time during the electroporation procedure.
Irreversible electroporation (IRE) is an emerging nonthermal technique that delivers short high-voltage electrical fields to puncture cell membranes and induce apoptosis.
In molecular cloning, Plasmid transformation into the bacterial competent cells by using electroporation has been found an efficient technique (Ryu and Hartin, 1990).
But existing electroporation methods require high electric field strengths and for cells to be suspended in solution, which disrupts cellular pathways and creates a harsh environment for sensitive primary cells.
According to the company, the new allowed patent application joins the patent US 8,026,223, which claims a method of treating cancerous tumors with plasmid-encoded IL-12 and electroporation therapy to the tumor; and patent US 8,802,643, which claims a method of treating cancerous tumors with plasmid-encoded B7-1, a peripheral membrane protein found on antigen-presenting cells (APCs) and electroporation therapy to the tumor, both of which have a field strength of at least 700 V/cm.
Electroporation is now being widely used to transfer a variety of macromolecules, including DNA, RNA, protein, and some chemotherapeutic agents, into cells (Miller and Nickoloff, 1995).
Electroporation is a form of drug delivery that generates electrical pulses via an electrode placed in a solid tumor to enhance the ability of a chemotherapeutic.
They cover amperometric biosensors, imaging of single biomolecules by scanning tunneling microscopy, applications in reflectivity in bioelectrochemistry, model lipid bilayers at electrode surfaces, enzymatic fuel cells, Raman spectroscopy of biomolecules at electrode surfaces, membrane electroporation in high electric fields, and electroporation for medical use in drug and gene electrotransfer.
The method Lu is referring to is called electroporation, a phenomenon known for decades that increases the permeability of a cell by applying an electric field to generate tiny pores in the membrane of cells.
Electroporation protocols; experimental and clinical gene medicine.
Known as irreversible electroporation, the technique uses carefully applied electrodes to deliver a brief, high-voltage current, which destabilizes the polarity of tumor cell membranes, without generating substantial heat.