eluant


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eluant

[′el·yə·wənt]
(chemistry)
A liquid used to extract one material from another, as in chromatography.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
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In order to examine the practical value of HCl as an eluant, the elution rate in 0.5 mol/L HCl was determined, using the same method above, and found to fast with t1/2 equal to 18 min.
The crude product was purified by flash chromatography, using dichloromethane as eluant, to give 5 as a dark solid (77.4 mg; yield 70%).
Sample pH and eluant were proved to be crucial parameters for antibiotics preconcentration using SPE (14).
The eluant was dried, resuspended in acetonitrile, and analyzed by LC-MS/MS on a Waters Acquity TQD system with an Acquity UPLC (HSS C18 UPLC column, 2.1 X 50 mm, 1.8 [micro]m) and an electrospray ionization source, which was used in positive ion mode.
As speciation of the 1% HN[O.sub.3] extracts was determined by ion chromatography coupled to ICP-MS using a Hamilton PRP X100 anion exchange column (Hamilton Company, Reno, NV) and a 20 mM ammonium phosphate eluant at pH 8.
The mixture was incubated at 37[degrees]C for 16 h and then analyzed by ITLC, using a silica gel-impregnated glass fiber 10-cm strip (ITLC-SG; Gelman) and an eluant of 1% DTPA (pH 6.0).
Yield: 98% eluant dichloromethane Rf=0.95 (CH2Cl2/MeOH 95/0.5); H-NMR: 7.23 (m, 5 H phenyl), 6.60 (d, 1H, H3, J CH=CH: 15.9), 6.24 (d 1H, H2, J2,3: 15.9, J2,1: 6.6), 4.68 (d 2H, H1, J1: 5.4), 2.02 (S, 3H, CH3).
Total RNA was extracted from swab eluant and amplified by using an M gene real-time reverse transcription PCR (RT-PCR) capable of detecting pandemic (H1N1) 2009 virus (9); 4 of 6 swabs were positive.
The complexity of the matrix means that even after extraction, there are still endogenous components remaining within the eluant, although these levels are dramatically reduced following the solid phase extraction process.
Data were not collected during the exclusion (5 minutes); thereafter, ionizable material was recorded as a function of time, and m/z signals were used for eluant identification by comparison with Wiley275 and Nist02 libraries.
The active eluant was collected and dialyzed against polyethylene glycol.
The average molecular weight and molecular weight distribution were determined by a gel permeation chromatography (GPC) instrument (Waters Breeze, USA) with tetrahydrofuran (THF) as an eluant and narrow polystyrene as the calibration standard.