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Mobile phase compositions are changed systemic by variation of the ACN content from 20% to 90% (v/v); the concentration of the eluent from 20 to 100 mM and its pH from 3.
Therefore, analysis of hardly-soluble polymers is usually conducted with general GPC device and hexafluoroisopropanol (HFIP) as an eluent.
Eluent pH is a particularly important factor to consider when analyzing ionizable compounds to ensure these analytes remain in their desired ionization state.
998 for acetonitrile-containing eluent and methanol-containing eluent, respectively).
Desorption percentage of RhB from EMDN surface was found to be low for all the desorbing eluents used and HCl has the highest desorption percentage of 17.
When reconstituted, typically with eluent from the purification stage, the sugars must not inhibit the PCR reaction.
The type and concentration of eluent is also important for the analytical parameters of a column solid-phase pre-concentration system.
Reversed-phase HELC in eluents of the acetonitrile-formic acid-water system was used in the isocratic modein eluents from 6 to 10 vol.
40 mL of the eluent were transferred to a suitable vessel containing a sample disk.
After an overnight incubation, 50 [micro]L of each DBS eluent was added to the same filter plate.
1% phosphoric acid aqueous solution (eluent A) and acetonitrile (eluent B) with the following gradient program: 30% eluent B in 20 min; 30-50% eluent B from 20 min to 35 min, and 50% eluent B from 35 min to 50 min.
01 M Phosphate buffer of pH 7 and acetonitrile as eluent at the detection wavelength of 290 nm.
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