To assess the limit of detection (LOD), 50 mL of a blank solution percolated via SPE column and then eluted with the elution
solution described above.
The methods for the elution
of the samples have also been largely empirical and therefore need to be standardized to maximize the performance of specific tests.
In this initial international study, subjects were randomized to receive either one of two models of the implant, each with different elution
rates, or topical travoprost.
Since the system measures the UV absorbance of the sample during purification, the target protein can be identified by an increase in UV absorbance with reduced glutathione during elution
. The purified protein was collected in 200 [micro]L fractions.
The samples are then transferred from the wash buffer into the elution
buffer, where the labelled probes dissociate from the transcript-probe complex.
In the IFCC reference measurement procedure for determination of Hb[A.sub.1c], the HPLC-ESI/MS analysis makes use of a cyanopropyl column and a TFAcontaining elution
Key words: Antimutagen, Anticarcinogen, Phyllanthus amarus, Plant, Alkaline elution
, Mutation assay
luteus endonuclease-sensitive lesions by alkaline elution
. Mutat Res 94:263-276 (1982).
To study the elution
behavior and the effect that the components of the culture medium produce in the retention of the polymers, the following solutions were analyzed at 35 [degrees] C using water as the mobile phase at a flow rate of 0.8 ml/min.
times for L-dopa and dopamine under these conditions were 7.82 [+ or -] 0.08 (SD; n = 19) and 13.25 [+ or -] 0.08 min (SD; n = 18), respectively.
Unlike in-gel digestion, in which some peptides never leave the gel, the whole protein sample is electrophoresed into the elution