The lanes of the agarose gel contains reaction products of DNA contacted with either no-coating (lane 1); Minwax without any bio-additive (lanes 2-4); Minwax functionalized with EcoRI endonuclease
(lanes 5-7); or Minwax functionalized with DNase I (lanes 8-10).
Charpentier, "A programmable dual-RNA-guided DNA endonuclease
in adaptive bacterial immunity," Science, vol.
has two independent Ruvc and HNH domains.
To further confirm the absence of any cross-reaction, experiments were individually performed in the presence of the reagents except for GoTaq DNA polymerase or Afu flap endonuclease
This resulted in the creation of a synthetic, chimeric sequence-specific nuclease genetic construct containing the DNA-binding domain of TALEs and the catalytic domain of FokI restriction endonuclease
. This construct helped to create artificial nucleases with DNA-binding domain and different RVDs that can target any nucleotide sequence of interest [2, 4].
Trotta, "Identification of a human endonuclease
complex reveals a link between tRNA splicing and pre-mRNA 3' end formation," Cell, vol.
Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease
The introduction of an unpaired base (A-C) at position 4 of the forward primer, located near the mutation site, allowed for establishment of an endonuclease
BamHI restriction site.
VIII-like 1 (NEIL1) promotes short-term spatial memory retention and protects from ischemic stroke-induced brain dysfunction and death in mice.
To examine whether TALEN could be used for the targeted cleavage of endogenous Sall1 in porcine fibroblasts, genomic DNA isolated from fibroblasts transfected with the TALEN expression vector was treated with T7 endonuclease
I, which specifically cleaves heteroduplexes formed by the hybridization of wild-type and mutant DNA sequences.
Thus, some authors believe that the endonuclease
G released from mitochondria initiates DNA fragmentation and thereafter, this process of fragmentation is increased by DNase I released from necrotic cells.
To resolve these cases, the amplified product was also digested with AflIII restriction endonuclease
. However, even with this enzymatic digestion it is not possible to further distinguish HPV70 from HPV85.