as a Biomarker: Biochemical and Clinical Aspects.
Yokoyama et al., "Overexpression of alpha enolase
in hepatitis C virus-related hepatocellular carcinoma: association with tumor progression as determined by proteomic analysis," Proteomics, vol.
Yandi, "The prognostic value of neuron-specific enolase
in head trauma patients," The Journal of Emergency Medicine, vol.
Immunoreactivity is variously positive for keratin antibodies (cytokeratin, Ck7, CK20, and CAM5.2) and neuroendocrine markers (chromogranin, synaptophysin, serotonin, nonspecific enolase
, and human pancreatic polypeptide) [6, 12].
((d), (e)) Tumor cells are positive for synaptophysin and neuron-specific enolase
The extracted HEK-293 cells and human pituitary protein samples as well as gamma enolase
recombinant proteins (#N2175; US Biological, Salem, MA, USA) were subjected to denaturing electrophoresis in 12% SDS-PAGE gels.
(2-phospho-D-glycerate hydrolase, EC 126.96.36.199) is a metalloenzyme that catalyzes the reversible dehydration of D-2-phosphoglycerate (PGA) to phosphoenolpyruvate (PEP) in both glycolysis and gluconeogenesis.
The laboratory reports showed elevated serum level of erythrocyte sedimentation rate (25.00 mm/hour), neuron-specific enolase
(NSE, 17.73 ng/mL, reference range 0-16.3 ng/mL), cytokeratin 19 fragment (CYFRA 21-1, 8.87 ng/mL, reference range 0-3.3 ng/mL), while other measured laboratory data were within normal limits, including serum leukocyte and antimycobacterium tuberculosis antibody.
The cells of SPNs demonstrate strong positive staining for [beta]-catenin, CD10, vimentin, [alpha]-1-antitrypsin, [alpha]-1-antichymotrypsin, neuron-specific enolase
, and cyclin D1 (Figure 2, A through C).5,16 Neoplastic cells harbor somatic point mutations in exon 3 of CTNNB1, the gene encoding [beta]-catenin in Wnt signaling pathway, resulting in nuclear condensation of [beta]-catenin and cyclin D1, and also loss of E-cadherin receptors on cellular membranes.
The membrane was blocked and blots were intubated in a specific antibody against enolase
superfamily member 1 (ENOSF1) and transferrin, followed by secondary antibodies according to manufacturer.
Axons stain with neuron specific enolase
and neurofilament proteins.
From water insoluble fraction, vimentin, gelsolin, gamma enolase
like protein, filensin like protein and ATP synthase subunit B were identified.